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Bovine Serum Albumin

SIGMA/A3733 - lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym: Albumin bovine serum; BSA; Bovine albumin

CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A3733-10G 10 g
$102.00
1/EA
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45-A3733-50G 50 g
$245.00
1/EA
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45-A3733-100G 100 g
$448.00
1/EA
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45-A3733-500G 500 g
$1360.00
1/EA
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45-A3733-1KG 1 kg
$1860.00
1/EA
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Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3733-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3733-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3733-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A3733-10G

 

assay ≥98% (agarose gel electrophoresis)
biological source bovine
form lyophilized powder
mol wt ~66 kDa
origin New Zealand origin
packaging poly bottle of
pH 7
  ~7 (1% in 0.15 M NaCl)
purified by heat shock fractionation
Quality Level 200 
solubility water: slightly soluble
storage temp. 2-8°C
technique(s) flow cytometry: suitable
UniProt accession no. P02769 
Application: Bovine Serum Albumin has been used:
• as a component of cryomedia for freezing testicular tissue
• to resuspend myometrial cells in flow cytometric analysis for cell viability quantification
• to block cardiac fibroblasts (CFs) for immunofluorescence staining

Biochem/physiol Actions: Bovine Serum Albumin (BSA) is a transporter for drugs, hormones, and fatty acids. It displays high solubility and is exploited in the cosmetic and pharmaceutical industries. BSA is useful as a cell media component and favors embryonic stem cells (hESC)differentiation.
Biochem/physiol Actions: Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
General description: Bovine serum albumin (BSA) that belongs to the serum albumin family, is an α-helical, globular and non-glycosylated protein. It comprises three domains with two subdomains under each domain.
Packaging: 1 kg in poly drum
Packaging: 10, 50, 100, 500 g in poly bottle
Preparation Note: Derived from New Zealand source serum
Preparation Note: Prepared using heat shock fractionation
Preparation Note: Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (agarose gel electrophoresis)
Storage Temp. 2-8°C
UNSPSC 12352202

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