Bovine Serum Albumin
SIGMA/A3733 - lyophilized powder, ≥98% (agarose gel electrophoresis)
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥98% (agarose gel electrophoresis) |
biological source | bovine |
form | lyophilized powder |
mol wt | ~66 kDa |
origin | New Zealand origin |
packaging | poly bottle of |
pH | 7 |
~7 (1% in 0.15 M NaCl) | |
purified by | heat shock fractionation |
Quality Level | 200 |
solubility | water: slightly soluble |
storage temp. | 2-8°C |
technique(s) | flow cytometry: suitable |
UniProt accession no. | P02769 |
Application: | Bovine Serum Albumin has been used: • as a component of cryomedia for freezing testicular tissue • to resuspend myometrial cells in flow cytometric analysis for cell viability quantification • to block cardiac fibroblasts (CFs) for immunofluorescence staining |
Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is a transporter for drugs, hormones, and fatty acids. It displays high solubility and is exploited in the cosmetic and pharmaceutical industries. BSA is useful as a cell media component and favors embryonic stem cells (hESC)differentiation. |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
General description: | Bovine serum albumin (BSA) that belongs to the serum albumin family, is an α-helical, globular and non-glycosylated protein. It comprises three domains with two subdomains under each domain. |
Packaging: | 1 kg in poly drum |
Packaging: | 10, 50, 100, 500 g in poly bottle |
Preparation Note: | Derived from New Zealand source serum |
Preparation Note: | Prepared using heat shock fractionation |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% (agarose gel electrophoresis) |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |