Anti-PIAS2 (AB1) antibody produced in rabbit
SIGMA/AV36929 - affinity isolated antibody
Synonym: Anti-6330408K17Rik; Anti-AI462206; Anti-ARIP3; Anti-AU018068; Anti-Dib; Anti-Miz1; Anti-PIASxα; Anti-Protein inhibitor of activated STAT 2
Product Type: Chemical
Immunohistochemistry Anti-PIAS2 (ab1): Cat. No. AV36929: Immunohistochemistry of Pias2 in Mouse pancreas tissue with Pias2 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: NIH/3T3 (Cat. No. AV36929). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type NIH/3T3
Immunoblotting PIAS2 Antibody: Cat. No. AV36929: Western Blot analysis of PIAS2 in Mouse Pancreas cell lysates with PIAS2 Antibody at 1.0 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 54 kDa |
| NCBI accession no. | NP_032628   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, horse, human, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q8C5D8 |
| Biochem/physiol Actions: | Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. It plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It seems to be mostly involved in gene silencing. It binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of mouse Pias2 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: LSLIPVDPQYCPPMFLDSLTSPLTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |