Bovine Serum Albumin
SIGMA/B4287 - heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥98% |
biological source | bovine |
foreign activity | Protease free |
form | lyophilized powder |
grade | for molecular biology |
mol wt | ~66 kDa |
origin | USA origin |
packaging | poly bottle of |
pH | 7 |
product line | BioReagent |
purified by | heat shock fractionation |
Quality Level | 200 |
solubility | water: soluble (40 mg/ml) |
storage temp. | 2-8°C |
technique(s) | electrophoresis: suitable |
ELISA: suitable | |
western blot: suitable | |
UniProt accession no. | P02769 |
Application: | Select the right albumin for your application with the Albumin Selection Guide |
Application: | A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications. |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
Features and Benefits: | • Protease free • Suitable for blocking non-specific protein binding sites in hybridizations • Heat shock fractionated |
Packaging: | 1, 5, 25 g in poly bottle |
Preparation Note: | Prepared using heat shock fractionation |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |