p300 (C1135-2414) human
SIGMA/SRP2086 - recombinant, expressed in insect cells, ≥70% (SDS-PAGE)
Synonym: KAT3B; RP1-85F18.1; RSTS2; p300
Product Type: Chemical
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| color | clear colorless |
| concentration | 350 μg/mL |
| form | frozen liquid |
| mol wt | ~145 kDa |
| NCBI accession no. | NM_001429   |
| packaging | pkg of 4 μg |
| recombinant | expressed in insect cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| UniProt accession no. | Q09472 |
| Biochem/physiol Actions: | Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as a tumor suppressor. The C-terminus of p300 contains a histone acetyltransferase (HAT) domain expanded from residues 1195 to 1673, an activation domain (residues 1763-1812) that interacts with many transcription factors and an SRC-1 domain further down stream of the activation domain. P300 is able to acetylate histones and many other transcription factors, including p53, E2F, TFIIE, and TFIIF etc. implicating diverse roles of p300 in gene regulation. |
| General description: | E1A binding protein p300 (p300) has a central bromodomain and three cysteine- and histidine-rich regions. The carboxy-terminal region interacts with the adenovirus oncoprotein E1A. The gene encoding this protein is localized on human chromosome 22q13. |
| Physical form: | Clear and colorless frozen liquid solution |
| Preparation Note: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |