Renin from mouse
SIGMA/SRP6266 - recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
Synonym: CYM; CYMP; Chymosin; RENNIN
Product Type: Chemical
Immunoblotting Renin, mouse Cat. No. SRP6266,Mouse recombinant Renin.
| assay | ≥95% (SDS-PAGE) |
| biological source | mouse |
| form | lyophilized powder |
| impurities | <1 EU/μg endotoxin (LAL test) |
| mol wt | calculated mol wt 43.2 kDa |
| observed mol wt 45-55 kDa by SDS-PAGE (reducing) (Protein migrates due to glycosylation. Leu24 is the predicted N-terminus.) | |
| packaging | pkg of 10 μg |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| tag | 6-His tagged (C-terminus) |
| UniProt accession no. | P06281   |
| Biochem/physiol Actions: | Renin plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. It activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by angiotensin-converting enzyme (ACE), the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through β-1 receptor activation. It can bind to ATPase H+ transporting accessory protein 2 (ATP6AP2), which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, it’s binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of hydroxyacyl-CoA dehydrogenase/3-ketoacyl- |
| General description: | Renin, also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body′s renin-angiotensin system (RAS). The gene encoding it is localized on mouse chromosome 1. |
| Physical form: | Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
| Reconstitution: | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |