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Corning® Islet Solutions and Reagents

By Corning®

Islet cell isolation and processing for diabetes research requires a variety of specialized cell culture media, separation and gradient solutions, porcine-specific solutions, and related isolation, trimming, and storage/preservation solutions. Most islet solutions have been customized to include or leave out components such as insulin, transferrin, HSA, cipro-floxacin, and HEPES.


Corning’s portfolio of specialized media has been cited in multiple islet cell isolation proto-cols for type 1 diabetes research.

Islet Solutions and Reagents

         
Cat.No. Name EA    
23-98-021-CV Miami medium #1A with HSA and sodium bicarbonate $0.00
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23-98-304-CV CMRL 1066, supplemented, CIT modification $0.00
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23-99-603-CV CMRL 1066, supplemented $0.00
1/EA
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23-99-663-CV CMRL 1066 without phenol red, L-glutamine $0.00
1/EA
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23-99-595-CM RPMI 1640 with L-glutamine $0.00
1/EA
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23-99-597-CM HBSS (Hank's Balanced Salt Solution), modified for islets $0.00
1/EA
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23-99-674-CM Gradient stock solution, density 1.110 to 1.121 g/cm3 $0.00
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23-99-676-CM Trimming solution $0.00
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23-99-677-CM Cold storage/purification stock solution, density 1.026 to 1.032 g/cm3 $0.00
1/EA
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23-99-678-CM Phase I solution $0.00
1/EA
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23-99-723-CM Penta starch, 10% solution $0.00
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23-99-781-CV Perfusion solution with HEPES, without phenol red $0.00
1/EA
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23-99-782-CM Priming solution with HEPES, without phenol red, and sodium bicarbonate $0.00
1/EA
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23-99-783-CM Dilution solution, RPMI 1640 with HEPES, L-glutamine, without HSA and phenol red $0.00
1/EA
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23-99-784-CM Wash solution, medium 199 with 25 mM HEPES, without phenol red,
L-glutamine, and sodium bicarbonate
$0.00
1/EA
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23-99-785-CV Final wash/culture medium, CMRL 1066 without phenol red, with HSA, HEPES, and L-glutamine $0.00
1/EA
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23-99-786-CV Functionality/viability solution, CMRL 1066 without glucose $0.00
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Cell Separation and Gradient Solutions

         
Cat.No. Name EA    
23-99-662-CVS Stock Polysucrose solution (Euro-Collins), density 1.132 g/cm3 $0.00
1/EA
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23-99-690-CIS Islet gradient, density 1.037 g/cm3 $0.00
1/EA
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23-99-691-CIS Islet gradient, density 1.096 g/cm3 $0.00
1/EA
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23-99-692-CIS Islet gradient, density 1.108 g/cm3 $0.00
1/EA
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23-99-815-CIS Islet gradient, density 1.069 g/cm3 $0.00
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Porcine Solutions

         
Cat.No. Name EA    
23-98-343-CV Neonatal porcine islet culture medium $0.00
1/EA
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23-99-601-CM Medium 199, porcine modification, with 25 mM HEPES, without phenol red, L-glutamine, and sodium bicarbonate $0.00
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Preservation Solutions

         
Cat.No. Name EA    
23-99-408-CM Glucose solution (Euro-Collins) $0.00
1/EA
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23-99-409-CI Electrolyte additive solution (Euro-Collins) $0.00
1/EA
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