Corning® BioCoat™ Intestinal Epithelium Differentiation Environment
By Corning® BioCoat®
An integrated system designed to create in vitro intestinal models. Contains a specially formulated serum-free medium, culture supplements, sodium butyrate, and Corning BioCoat™ Fibrillar Collagen Cell Culture Inserts.
An in vitro system used for rapid differentiation of Caco-2 cells:
- Facilitates confluent enterocyte monolayer formation with barrier function within 72 hours
- Promotes barrier function with TEER > 150 ohms cm2 and mannitol permeability coefficient < 4x10-6 cm/sec
- Permits testing of both actively and passively transported compounds
- Allows for studies two to three days postseeding, 17-18 days earlier than with commonly used procedures
- Serum-free environment maximizes control over experimental conditions
- Saves labor time and media by eliminating three to five feedings
Complete protocol provides:
- Standardized procedures
- Capability for immediate start-up
Applications:
Caco-2 cells can be used as in vitro models of intestinal epithelial cells for the following:
- Examination of transepithelial passage of orally administered drugs to help predict bioavailability of new therapeutic agents in humans
- Studies of intestinal transport mechanisms for various nutrients, metabolites and trace elements, and of compounds that interfere with this process
- Investigation of the mechanisms involved in the entry, replication and expression of infectious agents of the intestinal tract
- Studies of structural and/or functional changes in the intestinal epithelium associated with diseases, such as Crohn’s disease, rheumatoid arthritis or drug-induced intestinal inflammation