Corning® BioCoat™ Smooth Muscle Cell Differentiation Environment
By Corning® BioCoat®
An in vitro system optimized for the differentiation of human and rat SMC. Includes specially formulated Corning BioCoat™ Growth Factor Reduced (GFR) Matrigel™ Matrix Cellware and serum-free Smooth Muscle Differentation Medium.
- Three-dimensional matrix promotes differentiated (contractile) SMC phenotype, low proliferative index, nodular morphology and ultrastructure dominated by microtubules and microfilaments
- Expression of cytoskeletal markers, α-smooth muscle actin, heavy chain myosin, and 150 kD h-caldesmon
- Carry out studies of SMC specific function two weeks earlier than Collagen I
- Reduced levels of growth factors for cytokine studies
- Serum-free environment maximizes control over experimental conditions
Complete protocol provides:
- Standardized procedures
- Capability for immediate start-up
Applications:
Corning Matrigel™ Matrix has been used to culture SMC for studies of:
- SMC differentiation
- Modulation of SMC function
- Molecular mechanisms of phenotypic transitions
- Effects of various compounds on SMC behavior
- Effects of ECM proteins on vascular SMC phenotype