Corning® Ham's F-12 Medium
By Corning®
Shipped at 15 to 30°C; store at 2 to 8°C.
Ham's F-12 Medium Formulation
| Catalog No. | 23-10-080 1X, Liquid Ham's F-12 | 23-50-040 Powder Ham's F-12 |
|---|---|---|
| Inorganic Salts | ||
| CaCl2 (anhydrous) | 33.30mg/L | 33.30mg/L |
| CuSO4 (anhydrous) | 0.0016 | 0.0016 |
| FeSO4•7H2O | 0.834 | 0.834 |
| KCl | 223.60 | 223.60 |
| KH2PO4 | - | - |
| MgSO4 (anhydrous) | 72.20 | 72.20 |
| NaCl | 7600.00 | 7600.00 |
| Na2HPO4 (anhydrous) | 142.00 | 142.00 |
| NaHCO3 | 1176.00 | - |
| ZnSO4•7H2O | 0.863 | 0.863 |
| Amino acids | ||
| L-Alanine | 8.90 | 8.90 |
| L-Arginine•HCl | 211.00 | 211.00 |
| L-Asparagine•H2O | 15.00 | 15.00 |
| L-Aspartic acid | 13.30 | 13.30 |
| L-Cysteine•HCl•H2O | 35.12 | 35.12 |
| L-Glutamic acid | 14.70 | 14.70 |
| L-Glutamine | 146.20 | 146.20 |
| Glycine | 7.50 | 7.50 |
| L-Histidine•HCl•H2O | 20.96 | 20.96 |
| L-Isoleucine | 3.94 | 3.94 |
| L-Leucine | 13.10 | 13.10 |
| L-Lysine•HCl | 36.50 | 36.50 |
| L-Methionine | 4.48 | 4.48 |
| L-Phenylalanine | 4.96 | 4.96 |
| L-Proline | 34.50 | 34.50 |
| L-Serine | 10.50 | 10.50 |
| L-Threonine | 11.90 | 11.90 |
| L-Tryptophan | 2.04 | 2.04 |
| L-Tyrosine•2Na•2H2O | 7.84 | 7.84 |
| L-Valine | 11.70 | 11.70 |
| Vitamins | ||
| Biotin | 0.0073 | 0.0073 |
| D-Calcium pantothenate | 0.48 | 0.48 |
| Choline chloride | 13.96 | 13.96 |
| Folic acid | 1.30 | 1.30 |
| i-Inositol | 18.02 | 18.02 |
| Nicotinamide | 0.037 | 0.037 |
| Pyridoxine•HCl | 0.062 | 0.062 |
| Riboflavin | 0.038 | 0.038 |
| Thiamine•HCl | 0.34 | 0.34 |
| Vitamin B12 | 1.36 | 1.36 |
| Other | ||
| D-Glucose | 1802.00 | 1802.00 |
| Hypoxanthine, Na | 4.77 | 4.77 |
| Lipoic acid | 0.21 | 0.21 |
| Methyl lineoleate | 0.088 | 0.088 |
| Phenol red, Na | 1.20 | 1.20 |
| Putrescine•2HCl | 0.16 | 0.16 |
| Sodium pyruvate | 110.00 | 110.00 |
| Thymidine | 0.73 | 0.73 |
| Add | ||
| NaHCO3 Powder 7.5% Solution | - - | 1.176g/L 15.70mL/L |
| Specifications | ||
| pH (before buffer) | N/A | 6.5±0.5 |
| pH (after buffer) | 7.2±0.2 | 7.5±0.5 |
| Osmolality (mOsm) | 290±30 | 290±30 |
| Grams of powder required to prepare 1L (1X Solution) | N/A | 10.64 |