Corning® McCoy's 5A (Iwakata & Grace Modification)
By Corning®
Shipped at 15 to 30°C; store at 2 to 8°C.
McCoy's 5A Medium Formulation
| Catalog No. | 23-10-050 1X, Liquid McCoy's 5A | 23-10-051 1X, Liquid McCoy's 5A | |
| Inorganic Salts | |||
| CaCl2 (anhydrous) | 100.00mg/L | 100.00mg/L | |
| KCl | 400.00 | 400.00 | |
| MgSO4 (anhydrous) | 97.70 | 97.70 | |
| NaCl | 6460.00 | 5960.00 | |
| NaH2PO4•H2O | 580.00 | 580.00 | |
| NaHCO3 | 2200.00 | 2200.00 | |
| Amino acids | |||
|---|---|---|---|
| L-Alanine | 13.36 | 13.36 | |
| L-Arginine•HCl | 42.14 | 42.14 | |
| L-Asparagine•H2O | 45.03 | 45.03 | |
| L-Aspartic acid | 19.97 | 19.97 | |
| L-Cysteine•HCl•H2O | 35.14 | 35.14 | |
| L-Glutamic acid | 22.10 | 22.10 | |
| L-Glutamine | 219.20 | 219.20 | |
| L-Glycine | 7.50 | 7.50 | |
| L-Histidine•HCl•H2O | 20.96 | 20.96 | |
| Hydroxy-L-proline | 19.70 | 19.70 | |
| L-Isoleucine | 39.36 | 39.36 | |
| L-Leucine | 39.36 | 39.36 | |
| L-Lysine•HCl | 36.54 | 36.54 | |
| L-Methionine | 14.92 | 14.92 | |
| L-Phenylalanine | 16.52 | 16.52 | |
| L-Proline | 17.30 | 17.30 | |
| L-Serine | 26.30 | 26.30 | |
| L-Threonine | 17.90 | 17.90 | |
| L-Tryptophan | 3.10 | 3.10 | |
| L-Tyrosine•2Na•2H2O | 26.12 | 26.12 | |
| L-Valine | 17.60 | 17.60 | |
| Vitamins | |||
| Ascorbic acid | 0.50 | 0.50 | |
| Biotin | 0.20 | 0.20 | |
| D-Calcium pantothenate | 0.20 | 0.20 | |
| Choline chloride | 5.00 | 5.00 | |
| Folic acid | 10.00 | 10.00 | |
| i-Inositol | 36.00 | 36.00 | |
| Nicotinamide | 0.50 | 0.50 | |
| Nicotinic acid | 0.50 | 0.50 | |
| Para-Aminobenzoic acid | 1.00 | 1.00 | |
| Pyridoxine•HCl | 1.00 | 1.00 | |
| Riboflavin | 0.20 | 0.20 | |
| Thiamine•HCl | 0.20 | 0.20 | |
| Vitamin B12 | 2.00 | 2.00 | |
| Other | |||
| Bacto-peptone | 600.00 | 600.00 | |
| D-Glucose | 3000.00 | 3000.00 | |
| Glutathione (reduced) | 0.50 | 0.50 | |
| HEPES | - | 5958.00 | |
| Phenol red, Na | 10.00 | 10.00 | |
| Add | |||
| NaHCO3 Powder 7.5% Solution | - - | - - | |
| Specifications | |||
| pH (before buffer) | N/A | N/A | |
| pH (after buffer) | 7.2±0.2 | 7.2±0.2 @ 20-25°C | |
| Osmolality (mOsm) | 290±30 | 305±30 | |
| Grams of powder required to prepare 1L (1X Solution) | N/A | N/A | |