Corning® Medium 199 (Mod.)
By Corning®
| Cat.No. | Description | Pack Size | Shipped | Storage | Intended Use |
|---|---|---|---|---|---|
| Medium 199 (Mod.), 1X | |||||
| 23-10-060-CV | with Earle's salts & L-glutamine | 6 x 500 mL | A | R | I |
| Medium 199 (Mod.), Powder | |||||
| 23-50-050-PB | with Earle's salts & L-glutamine without sodium bicarbonate | 1 x 10 L | A | R | IVD |
| 23-50-050-PC | with Earle's salts & L-glutamine without sodium bicarbonate | 1 x 50 L | A | R | IVD |
| 23-90-050-PB | with Earle's salts without sodium bicarbonate, L-glutamine, & phenol red | 1 x 10 L | A | R | IVD |
| 23-90-050-PC | with Earle's salts without sodium bicarbonate, L-glutamine, & phenol red | 1 x 50 L | A | R | IVD |
| 23-50-051-PB | with Hanks' salts & L-glutamine without sodium bicarbonate | 1 x 10 L | A | R | IVD |
| 23-50-051-PC | with Hanks' salts & L-glutamine without sodium bicarbonate | 1 x 50 L | A | R | IVD |
F = -5 to -20°C
R = 2 to 8°C
A = 15 to 30°C
* = Glass
** = Flexible Media Bag
I = Sterile, IVD Product
NI = Sterile, Research Use Only, Not for IVD Use
IVD = In Vitro Diagnostic
Res = Research Use Only, Not for IVD Use
Medium 199 Formulation
| Catalog No. | 23-10-060 1X, Liquid M199E | 23-50-050 Powder M199E | 23-50-051 Powder M199H | 23-90-050 Powder M199E |
|---|---|---|---|---|
| Inorganic Salts | ||||
| CaCl2 (anhydrous) | 200.00mg/L | 200.00mg/L | 140.00mg/L | 200.00mg/L |
| Fe(NO3)3•9H2O | 0.72 | 0.72 | 0.72 | 0.72 |
| KCl | 400.00 | 400.00 | 400.00 | 400.00 |
| KH2PO4 | - | - | 60.00 | - |
| MgSO4 (anhydrous) | 97.70 | 97.70 | 97.70 | 97.70 |
| NaCl | 6800.00 | 6800.00 | 8000.00 | 6800.00 |
| NaH2PO4•H2O | 140.00 | 140.00 | - | 140.00 |
| Na2HPO4 (anhydrous) | - | - | 47.50 | - |
| NaHCO3 | 2200.00 | - | - | - |
| Amino acids | ||||
| L-Alanine | 25.00 | 25.00 | 25.00 | 25.00 |
| L-Arginine•HCl | 70.00 | 70.00 | 70.00 | 70.00 |
| L-Aspartic acid | 30.00 | 30.00 | 30.00 | 30.00 |
| L-Cysteine•HCl•H2O | 0.11 | 0.11 | 0.11 | 0.11 |
| L-Cystine•2HCl | 26.00 | 26.00 | 26.00 | 26.00 |
| L-Glutamic acid | 75.00 | 75.00 | 75.00 | 75.00 |
| L-Glutamine | 100.00 | 100.00 | 100.00 | - |
| Glycine | 50.00 | 50.00 | 50.00 | 50.00 |
| L-Histidine•HCl•H2O | 21.88 | 21.88 | 21.88 | 21.88 |
| Hydroxy-L-proline | 10.00 | 10.00 | 10.00 | 10.00 |
| L-Isoleucine | 20.00 | 20.00 | 20.00 | 20.00 |
| L-Leucine | 60.00 | 60.00 | 60.00 | 60.00 |
| L-Lysine•HCl | 70.00 | 70.00 | 70.00 | 70.00 |
| L-Methionine | 15.00 | 15.00 | 15.00 | 15.00 |
| L-Phenylalanine | 25.00 | 25.00 | 25.00 | 25.00 |
| L-Proline | 40.00 | 40.00 | 40.00 | 40.00 |
| L-Serine | 25.00 | 25.00 | 25.00 | 25.00 |
| L-Threonine | 30.00 | 30.00 | 30.00 | 30.00 |
| L-Tryptophan | 10.00 | 10.00 | 10.00 | 10.00 |
| L-Tyrosine•2Na•2H2O | 57.87 | 57.87 | 57.87 | 57.87 |
| L-Valine | 25.00 | 25.00 | 25.00 | 25.00 |
| Vitamins | ||||
| DL-alpha-Tocopherol•PO4•Na | 0.01 | 0.01 | 0.01 | 0.01 |
| Ascorbic acid | 0.05 | 0.05 | 0.05 | 0.05 |
| Biotin | 0.01 | 0.01 | 0.01 | 0.01 |
| Calciferol | 0.10 | 0.10 | 0.10 | 0.10 |
| D-Calcium pantothenate | 0.01 | 0.01 | 0.01 | 0.01 |
| Choline chloride | 0.50 | 0.50 | 0.50 | 0.50 |
| Folic acid | 0.01 | 0.01 | 0.01 | 0.01 |
| i-Inositol | 0.05 | 0.05 | 0.05 | 0.05 |
| Menadione•NaHSO3•3H2O | 0.019 | 0.019 | 0.019 | 0.019 |
| Nicotinic acid | 0.025 | 0.025 | 0.025 | 0.025 |
| Nicotinamide | 0.025 | 0.025 | 0.025 | 0.025 |
| Para-Aminobenzoic acid | 0.05 | 0.05 | 0.05 | 0.05 |
| Pyridoxine•HCl | 0.05 | 0.05 | 0.05 | 0.05 |
| Riboflavin | 0.01 | 0.01 | 0.01 | 0.01 |
| Thiamine•HCl | 0.01 | 0.01 | 0.01 | 0.01 |
| Vitamin A acetate | 0.14 | 0.14 | 0.14 | 0.14 |
| Other | ||||
| Adenine sulfate | 10.00 | 10.00 | 10.00 | 10.00 |
| 5-Adenylic acid•H2O | 0.20 | 0.20 | 0.20 | 0.20 |
| ATP, 2Na•3H2O | 1.00 | 1.00 | 1.00 | 1.00 |
| Cholesterol | 0.20 | 0.20 | 0.20 | 0.20 |
| 2-Deoxy-D-ribose | 0.50 | 0.50 | 0.50 | 0.50 |
| D-Glucose | 1000.00 | 1000.00 | 1000.00 | 1000.00 |
| Glutathione (reduced) | 0.05 | 0.05 | 0.05 | 0.05 |
| Guanine•HCl | 0.30 | 0.30 | 0.30 | 0.30 |
| Hypoxanthine, Na | 0.354 | 0.354 | 0.354 | 0.354 |
| Phenol red, Na | 10.00 | 10.00 | 10.00 | - |
| D-Ribose | 0.50 | 0.50 | 0.50 | 0.50 |
| Sodium acetate | 50.00 | 50.00 | 50.00 | 50.00 |
| Thymine | 0.30 | 0.30 | 0.30 | 0.30 |
| Tween 80 | 20.00 | 20.00 | 20.00 | 20.00 |
| Uracil | 0.30 | 0.30 | 0.30 | 0.30 |
| Xanthine, Na | 0.34 | 0.34 | 0.34 | 0.34 |
| Add | ||||
| NaHCO3 Powder 7.5% Solution | - - | 2.20g/L 29.33mL/L | 0.35g/L 4.70mL/L | 2.20g/L 29.33mL/L |
| L-Glutamine Powder 200 mM Solution | - - | - - | - - | 100.00mL/L 3.40mL/L |
| Specifications | ||||
| pH (before buffer) | N/A | 4.5±0.5 | 4.5-6.0 | 4.5±0.5 |
| pH (after buffer) | 7.2±0.2 | 7.5±0.5 | 6.9±0.2 | 7.5±0.5 |
| Osmolality (mOsm) | 290±30 | 260-320 | 275-315 | 260-320 |
| Grams of powder required to prepare 1L (1X Solution) | N/A | 9.50 | 10.63 | 9.39 |