Corning® Minimum Essential Medium Alpha Medium (AMEM)
By Corning®
Shipped at 15 to 30°C; store at 2 to 8°C.
| Cat.No. | Description | Pack Size | Shipped | Storage | Intended Use |
|---|---|---|---|---|---|
| Minimum Essential Medium (MEM) Alpha Medium, 1X | |||||
| 23-10-022-CV | with Earle's salts, ribonucleosides, deoxyribonucleosides, & L-glutamine | 6 x 500 mL | A | R | I |
| 23-15-012-CV | with Earle's salts without ribonucleosides, deoxyribonucleosides, & L-glutamine | 6 x 500 mL | A | R | I |
| Minimum Essential Medium (MEM) Alpha Medium, Powder | |||||
| 23-50-012-PB | with Earle's salts & L-glutamine without ribonucleosides, deoxyribonucleosides, & sodium bicarbonate | 1 x 10 L | A | R | IVD |
| 23-50-012-PC | with Earle's salts & L-glutamine without ribonucleosides, deoxyribonucleosides, & sodium bicarbonate | 1 x 50 L | A | R | IVD |
| 23-90-012-PB | with Earle's salts without ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, & sodium bicarbonate | 1 x 10 L | A | R | IVD |
| 23-90-012-PC | with Earle's salts without ribonucleosides, deoxyribonucleosides, L-glutamine, phenol red, & sodium bicarbonate | 1 x 50 L | A | R | IVD |
F = -5 to -20°C
R = 2 to 8°C
A = 15 to 30°C
* = Glass
** = Flexible Media Bag
I = Sterile, IVD Product
NI = Sterile, Research Use Only, Not for IVD Use
IVD = In Vitro Diagnostic
Res = Research Use Only, Not for IVD Use
Minimum Essential Medium, Alpha Modification Formulation
| Catalog No. | 23-10-022 1X, Liquid AMEM | 23-15-012 1X, Liquid AMEM | 23-50-012 Powder AMEM | 23-90-012 Powder AMEM |
|---|---|---|---|---|
| Inorganic Salts | ||||
| CaCl2 (anhydrous) | 200.00mg/L | 200.00mg/L | 200.00mg/L | 200.00mg/L |
| KCl | 400.00 | 400.00 | 400.00 | 400.00 |
| MgSO4 (anhydrous) | 97.70 | 97.70 | 97.70 | 97.70 |
| NaCl | 6800.00 | 6800.00 | 6800.00 | 6800.00 |
| NaH2PO4•H2O | 140.00 | 140.00 | 140.00 | 140.00 |
| NaHCO3 | 2200.00 | 2200.00 | - | - |
| Amino acids | ||||
| L-Alanine | 25.00 | 25.00 | 25.00 | 25.00 |
| L-Arginine•HCl | 126.40 | 126.40 | 126.40 | 126.40 |
| L-Asparagine•H2O | 50.00 | 50.00 | 50.00 | 50.00 |
| L-Aspartic acid | 30.00 | 30.00 | 30.00 | 30.00 |
| L-Cysteine•HCl•H2O | 100.00 | 100.00 | 100.00 | 100.00 |
| L-Cystine•2HCl | 31.20 | 31.20 | 31.20 | 31.20 |
| L-Glutamic acid | 75.00 | 75.00 | 75.00 | 75.00 |
| L-Glutamine | 292.00 | - | 292.00 | - |
| Glycine | 50.00 | 50.00 | 50.00 | 50.00 |
| L-Histidine•HCl•H2O | 41.90 | 41.90 | 41.90 | 41.90 |
| L-Isoleucine | 52.50 | 52.50 | 52.50 | 52.50 |
| L-Leucine | 52.50 | 52.50 | 52.50 | 52.50 |
| L-Lysine•HCl | 72.50 | 72.50 | 72.50 | 72.50 |
| L-Methionine | 15.00 | 15.00 | 15.00 | 15.00 |
| L-Phenylalanine | 32.50 | 32.50 | 32.50 | 32.50 |
| L-Proline | 40.00 | 40.00 | 40.00 | 40.00 |
| L-Serine | 25.00 | 25.00 | 25.00 | 25.00 |
| L-Threonine | 47.60 | 47.60 | 47.60 | 47.60 |
| L-Tryptophan | 10.00 | 10.00 | 10.00 | 10.00 |
| L-Tyrosine•2Na•2H2O | 51.90 | 51.90 | 51.90 | 51.90 |
| L-Valine | 46.80 | 46.80 | 46.80 | 46.80 |
| Vitamins | ||||
| Ascorbic acid | 50.00 | 50.00 | 50.00 | 50.00 |
| Biotin | 0.10 | 0.10 | 0.10 | 0.10 |
| D-Calcium pantothenate | 1.00 | 1.00 | 1.00 | 1.00 |
| Choline chloride | 1.00 | 1.00 | 1.00 | 1.00 |
| Folic acid | 1.00 | 1.00 | 1.00 | 1.00 |
| i-Inositol | 2.00 | 2.00 | 2.00 | 2.00 |
| Nicotinamide | 1.00 | 1.00 | 1.00 | 1.00 |
| Pyridoxine•HCl | 1.00 | 1.00 | 1.00 | 1.00 |
| Riboflavin | 0.10 | 0.10 | 0.10 | 0.10 |
| Thiamine•HCl | 1.00 | 1.00 | 1.00 | 1.00 |
| Vitamin B12 | 1.36 | 1.36 | 1.36 | 1.36 |
| Other | ||||
| D-Glucose | 1000.00 | 1000.00 | 1000.00 | 1000.00 |
| Lipoic acid | 0.20 | 0.20 | 0.20 | 0.20 |
| Phenol red, Na | 10.00 | 10.00 | 10.00 | - |
| Sodium pyruvate | 110.00 | 110.00 | 110.00 | 110.00 |
| Nucleosides | ||||
| Thymidine | 10.00 | - | - | - |
| Adenosine | 10.00 | - | - | - |
| Cytidine | 10.00 | - | - | - |
| Guanosine | 10.00 | - | - | - |
| Uridine (anhydrous) | 10.00 | - | - | - |
| 2'-Deoxyadenosine | 10.00 | - | - | - |
| 2'-Deoxycytidine•HCl | 11.00 | - | - | - |
| 2'-Deoxyguanosine | 10.00 | - | - | - |
| Add | ||||
| NaHCO3 Powder (g/L) 7.5% Solution (mL/L) | - - | - - | 2.20 29.33 | 2.20 29.33 |
| L-Glutamine Powder (mg/L) 200 mM Solution (mL/L) | - - | 292.00 10.00 | - - | 292.00 10.00 |
| Specifications | ||||
| pH (before buffer) | N/A | N/A | 3.5±0.5 | 3.5±0.5 |
| pH (after buffer) | 7.2±0.2 | 7.2±0.2 | 7.0±0.5 | 7.0±0.5 |
| Osmolality (mOsm) | 290±15 | 290±30 | 290±30 | 290±30 |
| Grams of powder required to prepare 1L (1X Solution) | N/A | N/A | 10.09 | 9.78 |