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Spectrum® Chromatography Columns

Spectrum® Chromatography Columns

Selection of the chromatography column appropriate to a particular project depends on the capacity and degree of resolution required and on the pressure and nature of the eluent to which it will be subjected. In general, long columns are chosen for separations requiring high resolution; large diameter columns are used to handle large sample volumes; and small columns are used for ion exchange and affinity chromatography.

Spectrum® chromatography columns are available in diameters ranging from 0.9 to 10cm and in lengths from 15 to 150cm that can be joined by column extenders to form even longer units.

A full range of parts and optional accessories is available; contact Krackeler Scientific Customer Service for details.

Construction

Borosilicate columns are fitted with polypropylene collars and end plates for use with aqueous solutions or with PTFE ones for complete chemical resistance to organic solvents. Both are autoclavable.

  • Nonclogging 10mm bed support of nylon or PTFE membrane for aqueous and organic eluents suspended over a woven grid.
  • Upper end plates have side vent port for bleeding air or introducing sample.
  • Both top and bottom plates accept 3/16, 1/8 or 1/4" multifit tubing connectors or 1/4" PTFE multifit tubing.
  • Columns with diameter 5.0cm or less have threaded collars and end plates for screw-on connection.

*0.9cm I.D. columns are precision bore glass.

Columns with polypropylene end plates for use with aqueous solvent systems, 3/16" M-fit connectors

                 
Cat.No. Name I.D. x L Max. Pressure Bed Volume/cm Bed Volume Total EA    
265-124000 LC Column, Aqueous 0.9 x 15cm* 50 psi 0.6mL 9.4mL DISCONTINUED  
265-124002 LC Column, Aqueous 0.9 x 30* 50 0.6 19 DISCONTINUED  
265-124005 LC Column, Aqueous 0.9 x 60* 50 0.6 38 DISCONTINUED  
265-124006 LC Column, Aqueous 0.9 x 100* 50 0.6 63 DISCONTINUED  
265-124008 LC Column, Aqueous 1.5 x 20 45 1.7 35 $0.00
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265-124010 LC Column, Aqueous 1.5 x 30 45 1.7 53 DISCONTINUED  
265-124012 LC Column, Aqueous 1.5 x 40 45 1.7 68 DISCONTINUED  
265-124014 LC Column, Aqueous 1.5 x 60 45 1.7 106 DISCONTINUED  
265-124020 LC Column, Aqueous 1.5 x 100 45 1.7 177 DISCONTINUED  
265-124021 LC Column, Aqueous 2.5 x 20 40 4.9 98 $0.00
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265-124022 LC Column, Aqueous 2.5 x 30 40 4.9 147 DISCONTINUED  
265-124024 LC Column, Aqueous 2.5 x 40 40 4.9 196 DISCONTINUED  
265-124026 LC Column, Aqueous 2.5 x 45 40 4.9 216 DISCONTINUED  
265-124027 LC Column, Aqueous 2.5 x 50 40 4.9 245 DISCONTINUED  
265-124028 LC Column, Aqueous 2.5 x 60 40 4.9 294 DISCONTINUED  
265-124034 LC Column, Aqueous 2.5 x 100 40 4.9 490 DISCONTINUED  
265-124036 LC Column, Aqueous 5.0 x 30 25 19.6 588 $0.00
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265-124038 LC Column, Aqueous 5.0 x 60 25 19.6 1176 DISCONTINUED  

Columns with PTFE end plates for use with organic solvent systems, 1/8" M-fit connectors

                 
Cat.No. Name I.D. x L Max. Pressure Bed Volume/cm Bed Volume Total EA    
265-125001 LC Column, Organic 0.9 x 15cm* 50 psi 0.6mL 9.4mL DISCONTINUED  
265-125003 LC Column, Organic 0.9 x 30* 50 0.6 19 DISCONTINUED  
265-125005 LC Column, Organic 0.9 x 60* 50 0.6 38 DISCONTINUED  
265-125009 LC Column, Organic 1.5 x 20 45 1.7 35 $0.00
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265-125011 LC Column, Organic 1.5 x 30 45 1.7 53 DISCONTINUED  
265-125013 LC Column, Organic 1.5 x 40 45 1.7 68 DISCONTINUED  
265-125015 LC Column, Organic 1.5 x 60 45 1.7 106 $0.00
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265-125021 LC Column, Organic 1.5 x 100 45 1.7 177 DISCONTINUED  
265-125022 LC Column, Organic 2.5 x 20 40 4.9 98 DISCONTINUED  
265-125023 LC Column, Organic 2.5 x 30 40 4.9 147 DISCONTINUED  
265-125025 LC Column, Organic 2.5 x 40 40 4.9 196 DISCONTINUED  
265-125027 LC Column, Organic 2.5 x 45 40 4.9 216 DISCONTINUED  
265-125028 LC Column, Organic 2.5 x 50 40 4.9 245 DISCONTINUED  
265-125029 LC Column, Organic 2.5 x 60 40 4.9 294 DISCONTINUED  
265-125035 LC Column, Organic 2.5 x 100 40 4.9 490 DISCONTINUED  
265-125039 LC Column, Organic 5.0 x 60 25 19.6 1176 DISCONTINUED  

Columns with polypropylene end plates for use with aqueous solvent systems, 1/4" M-fit connectors

                 
Cat.No. Name I.D. x L Max. Pressure Bed Volume/cm Bed Volume Total EA    
265-124042 LC Column, Aqueous 5.0 x 100cm 25 psi 19.6mL 1960mL DISCONTINUED  
265-124044 LC Column, Aqueous 5.0 x 150 25 19.6 2940 DISCONTINUED  
265-124046 LC Column, Aqueous 7.5 x 30 15 44.2 1325 DISCONTINUED  
265-124047 LC Column, Aqueous 7.5 x 60 15 44.2 2650 DISCONTINUED  
265-124049 LC Column, Aqueous 7.5 x 100 15 44.2 4420 DISCONTINUED  
265-124052 LC Column, Aqueous 10 x 30 10 78.5 2355 DISCONTINUED  

Columns with PTFE end plates for use with organic solvent systems, 1/4" M-fit connectors

                 
Cat.No. Name I.D. x L Max. Pressure Bed Volume/cm Bed Volume Total EA    
265-125043 LC Column, Organic 5.0 x 100cm 25 psi 19.6mL 1960mL DISCONTINUED  
265-125045 LC Column, Organic 5.0 x 150 25 19.6 2940 DISCONTINUED  
265-125047 LC Column, Organic 7.5 x 30 15 44.2 1325 DISCONTINUED  
265-125048 LC Column, Organic 7.5 x 60 15 44.2 2650 DISCONTINUED  
265-125050 LC Column, Organic 7.5 x 100 15 44.2 4420 DISCONTINUED  
265-125053 LC Column, Organic 10 x 30 10 78.5 2355 DISCONTINUED  
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