Alkali-Cation™ Yeast Transformation Kit
By MP BiomedicalsHigh efficiency transformation of yeast cells
Transformation process complete in less than 90 minutes with efficiencies routinely greater than 104 transformants per µg plasmid DNA.
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two-hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation efficiencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
Kit Components: TE (pH 7.5), Lithium/Cesium Acetate, Carrier DNA, Histamine, PEG, TE/Cation MIXX, SOS