Bovine Serum Albumin solution
SIGMA/A1595 - 10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered
Synonym: Albumin solution from bovine serum; BSA solution
CAS Number: 9048-46-8
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
| biological source | bovine |
| concentration | 10% in DPBS |
| contains | does not contain stabilizer or preservative |
| form | solution |
| impurities | ≤0.05% fatty acid |
| ≤200 EU/mL endotoxin | |
| origin | USA origin |
| pH | 7.1-7.7 |
| product line | BioReagent |
| purified by | heat shock fractionation |
| Quality Level | 400  |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02769  |
| Application: | Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin. BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA. |
| Features and Benefits: | • Fatty acid free / tested to be low in fatty acid content • Low endotoxin • Sterile-filtered |
| Preparation Note: | Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652 . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |