Bovine Serum Albumin
SIGMA/A3059 - heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥98% |
biological source | bovine |
form | lyophilized powder |
impurities | essentially globulin free |
protease, none detected | |
loss | ≤5% |
mol wt | ~66 kDa |
origin | USA origin |
packaging | poly bottle of |
pH | 7 |
purified by | heat shock fractionation |
Quality Level | 300 |
solubility | water: soluble (40 mg/ml) |
storage temp. | 2-8°C |
technique(s) | ELISA: suitable |
western blot: suitable | |
UniProt accession no. | P02769 |
Application: | Bovine Serum Albumin has been used in luminescence assays. |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
Features and Benefits: | • Heat shock fractionated • Protease-free • Essentially globulin-free |
General description: | Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as: • Protein quantitation (as a standard) • Immunochemistry (as a blocking agent) • Cell culture (as a nutrient) This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like: • Enzyme assays • Protein-based assays • Protease-sensitive techniques that include: a) Enzyme immunoassay (EIA) b) Nucleic acid hybridization c) Radioimmunoassay (RIA) |
Packaging: | 10, 50, 100, 500 g in poly bottle |
Preparation Note: | Prepared using heat shock fractionation |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |