Bovine Serum Albumin
SIGMA/A7511 - lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥97% (agarose gel electrophoresis) |
biological source | bovine |
fatty acid content | ≤0.01% |
form | lyophilized powder |
mol wt | ~66 kDa |
origin | USA origin |
packaging | poly bottle of |
pH | 7 |
purified by | cold ethanol fractionation |
quality | crystallized |
Quality Level | 200 |
solubility | water: soluble (50 mg/ml) |
storage temp. | 2-8°C |
technique(s) | electrophoresis: suitable |
ELISA: suitable | |
western blot: suitable | |
UniProt accession no. | P02769 |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
Features and Benefits: | • Essentially fatty acid free • Crystallized |
Packaging: | 1, 5, 10, 25 g in poly bottle |
Preparation Note: | Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method |
Preparation Note: | Prepared from albumin (A4378) |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥97% (agarose gel electrophoresis) |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |