Bovine Serum Albumin
SIGMA/A7888 - heat shock fraction, suitable for RIA, pH 5.2, ≥96%
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7888-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7888-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7888-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A7888-500G
| assay | ≥96% |
| biological source | bovine |
| form | lyophilized powder |
| loss | ≤5% |
| mol wt | ~66 kDa |
| origin | USA origin |
| packaging | poly bottle of |
| pH | 5.2 |
| purified by | heat shock fractionation |
| quality | suitable for RIA |
| Quality Level | 200  |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
| suitability | suitable for insulin RIA procedures |
| technique(s) | blood typing: suitable |
| UniProt accession no. | P02769  |
| Application: | Bovine Serum Albumin has been used: • as a blocking agent in enzyme linked immunosorbent assay (ELISA)(154) • as a component of Dulbecco PBS medium for preantral follicles culture(155) • as a component of modified Tris-buffered medium (mTBM) for oocyte insemination(156) |
| Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is a transporter for drugs, hormones and fatty acids. It is a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. The cysteine and tyrosine residues of BSA have high affinity for metal binding. |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Caution: | May yield an insulin blank of up to 0.1 μunit per mg in certain procedures. |
| Features and Benefits: | • Heat shock fractionated • Suitable for radioimmunoassay (RIA) |
| General description: | Bovine serum albumin (BSA) is 66 kDa protein consisting of three domains with two subdomains under each. It is α-helical globular and a non-glycosylated protein. |
| Packaging: | 10, 50, 100, 500 g in poly bottle |
| Preparation Note: | Prepared using heat shock fractionation |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |