Bovine Serum Albumin
SIGMA/A8806 - fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8806-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A8806-1G
| ≤0.02% | |
| assay | ≥96% (agarose gel electrophoresis) |
| biological source | bovine |
| form | lyophilized powder |
| impurities | ≤0.02% fatty acid (GC) |
| ≤0.1 ng/mg endotoxin | |
| loss | ≤5% |
| mol wt | ~66 kDa |
| origin | USA origin |
| packaging | poly bottle of |
| pH | 4.8-7.5 |
| product line | BioReagent |
| purified by | cold ethanol fractionation |
| Quality Level | 300  |
| solubility | water: soluble (40 mg/ml) |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02769  |
| Application: | Select the right albumin for your application with the Albumin Selection Guide |
| Application: | Bovine Serum Albumin has been used in the solution prepared for digestion of tissues. It has been used as a blocking agent in cell spreading assay. Because BSA is a well-known carrier of fatty acids (FA), control over the particular fatty acids used to culture cell lines is important, for cell culture lines which are sensitive to particular fatty acids. Fatty acid-free BSA is a highly useful reagent for such cell culture experiments, to allow researchers to use such BSA as a carrier for their specific fatty acids specific to their cell culture system. This mitigates potential risks to the cells from FA′s that might be present in non-FA-free BSA. This BSA product is specifically tested for the following properties: • Fatty acid free / very low FA content • Low endotoxin level • Suitability to use in cell culture |
| Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
| Features and Benefits: | • Tested for use in cell culture • Fatty acid-free • Low endotoxin • Cold ethanol fractionated |
| General description: | Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating. BSA is a carrier protein for many biomolecules, such as fatty acids, amino acids, and steroids. For its carrier properties, BSA is also widely used in cell culture. BSA also serves antioxidant functions when used in cell culture. |
| Packaging: | 1, 5 g in poly bottle |
| Preparation Note: | Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. |
| Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥96% (agarose gel electrophoresis) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |