Bovine Serum Albumin solution
SIGMA/A9205 - 30% in saline, fatty acid free, aseptically filled
Synonym: Albumin solution from bovine serum; BSA solution
CAS Number: 9048-46-8
MDL Number: MFCD00130384
Product Type: Chemical
Molecular Artwork based on the crystal structure of Bovine Serum Albumin (PDB ID 3v03) showing the 6 color-coded helical subdomains assembled to form the heart-shaped quaternary structure of the BSA protein molecule.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
Copyright 2013 Sigma-Aldrich Co. LLC, courtesy of Dr. David Goodsell.
| ≤0.01% | |
| assay | 28-32% protein basis (biuret) |
| biological source | bovine |
| concentration | 30% in saline |
| does not contain | stabilizer or preservative |
| form | solution |
| origin | USA origin |
| purified by | cold ethanol fractionation |
| Quality Level | 200  |
| sterility | aseptically filled |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P02769  |
| Application: | Bovine Serum Albumin solution has been used: • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells • as a component of hepatocyte recovery medium • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos |
| Biochem/physiol Actions: | Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates. |
| General description: | Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin. |
| Preparation Note: | Initial fractionation by cold alcohol precipitation |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 28-32% protein basis (biuret) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |