Monoclonal Anti-EMD antibody produced in mouse
SIGMA/AMAB90562 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0203, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: LEMD5; STA
Product Type: Product-on-demand
Enhanced Validation-RNAi Knockdown Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probes 1 and 2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry Immunohistochemical staining of human skin shows strong nuclear membrane positivity in the epidermis cells.
Immunohistochemistry Immunohistochemical staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in the trophoblast.
Immunohistochemistry Immunohistochemical staining of human testis shows nuclear membrane immunoreactivity.
Immunohistochemistry Immunohistochemical staining of human kidney shows nuclear membrane positivity in renal tubules.
Immunofluorescence Immunofluorescence staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Western Blotting Lane 1: Marker [kDa] Lane 2: RT-4
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL0203, monoclonal |
| conjugate | unconjugated |
| enhanced validation | RNAi knockdown Learn more about Antibody Enhanced Validation  |
| Ensembl | human accession no. | ENSG00000102119  |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 2-10 μg/mL |
| immunohistochemistry: 1:5000- 1:10000 | |
| western blot: 1 μg/mL | |
| UniProt accession no. | P50402 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Recombinant protein corresponding to Emerin. Sequence ASSYSFSDLNSTRGDADMYDLPKKE Epitope Binds to an epitope located within the peptide sequence QSKGYNDDYY as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC |
| Linkage: | Corresponding Antigen APREST74058 . |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |