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Monoclonal Anti-EMD antibody produced in mouse

SIGMA/AMAB90562 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0203, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: LEMD5; STA

Product Type: Product-on-demand

Catalog Number PKG Qty. Price Quantity
45-AMAB90562-100UL 100 µL
$564.00
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Enhanced Validation-RNAi Knockdown Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probes 1 and 2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry Immunohistochemical staining of human skin shows strong nuclear membrane positivity in the epidermis cells.
Immunohistochemistry Immunohistochemical staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong nuclear membrane positivity in the trophoblast.
Immunohistochemistry Immunohistochemical staining of human testis shows nuclear membrane immunoreactivity.
Immunohistochemistry Immunohistochemical staining of human kidney shows nuclear membrane positivity in renal tubules.
Immunofluorescence Immunofluorescence staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Western Blotting Lane 1: Marker [kDa] Lane 2: RT-4

 

antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone CL0203, monoclonal
conjugate unconjugated
enhanced validation RNAi knockdown
Learn more about Antibody Enhanced Validation 
Ensembl | human accession no. ENSG00000102119 
form buffered aqueous glycerol solution
isotype IgG1
product line Prestige Antibodies® Powered by Atlas Antibodies
Quality Level 100 
shipped in wet ice
species reactivity human
storage temp. −20°C
target post-translational modification unmodified
technique(s) immunofluorescence: 2-10 μg/mL
  immunohistochemistry: 1:5000- 1:10000
  western blot: 1 μg/mL
UniProt accession no. P50402 
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