Anti-Bovine Albumin antibody produced in rabbit
SIGMA/B1520 - whole antiserum
Synonym: Anti-Albumin, bovine serum (BSA)
MDL Number: MFCD00162166
Product Type: Chemical
Immunoblotting Species serum was separated on SDS-PAGE and probed with 1:2,000 Rabbit Anti-Bovine Albumin (Cat. No. B1520). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Human serum 2. Goat serum
Immunoprecipitation Rabbit Anti-Bovine Albumin (Cat. No. B1520) was used to immunoprecipitate BSA from Fetal bovine serum (Cat. No. F7524). Detection antibody: Monoclonal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. B2901), followed by Anti-Mouse IgG-peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Positive control: total 5 μg BSA without IP 2. Negative control: 10 μL non-relevant antibody 3. 2.5 μL antibody 4. 1 μL antibody
ELISA Anti-Bovine Albumin antibody produced in Rabbit (Cat. No. B1520), followed by 1:5,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Bovine Albumin by indirect ELISA.
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: 1:200,000 |
| quantitative precipitin assay: 3.0 mg/mL |
| Application: | Anti-Bovine Albumin antibody produced in rabbit may be used for ELISA at a working dilution of 1:200,000. The antibody was used for in vitro labeling of albumin present in mammary tissue extract of lactating cow. It has also been used for the detection of albumin in nanoparticle using scanning transmission electron microscope. |
| Biochem/physiol Actions: | Albumin is the most abundant circulating proteins present in the serum that binds and transports long-chain fatty acid anions, detoxifies unconjugated bilirubin and transports thyroid and steroid hormones. Human serum albumin as a transporter, is considered as drug delivery vehicle for treating human diseases. Post-translational modifications like glycation and cysteinylation affects the binding functionality of serum albumin. High albumin protein diet has influence on hyperalbuminemia. Low levels of albumin leads to hypoalbuminemia, associated with decreased production and distribution. Hypoalbuminemia is a risk factor for the development of cardiovascular disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Serum albumin in humans is encoded by the gene mapped to human chromosome 4q13.3. Serum albumin is one of the abundant proteins in the blood and has a molecular weight of 66 kDa. Anti-Bovine Serum Albumin (BSA) was developed in rabbit using purified BSA from Cohn Fraction V as the immunogen. The antiserum has been treated to remove lipoproteins. Strong reactivity with BSA has been determined by immunoelectrophoresis (IEP). |
| Physical form: | Rabbit Anti-BSA is provided as a liquid containing 15 mM sodium azide as preservative. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |