Anti-BNIP3 antibody, Mouse monoclonal
SIGMA/B7931 - clone ANa40, purified from hybridoma cell culture
Synonym: Monoclonal Anti-BNIP3 antibody produced in mouse
MDL Number: MFCD03457547
Product Type: Chemical
Western Blotting JOURNAL CITATION: Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. By: McMillan, E. M., Paré, M. F., et al. in PLoS One, 2015. PubMed ID: 25799101 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0119382), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Extract of 293T cells transfected with human BNIP3 was separated on SDS-PAGE and probed with Monoclonal PA to BNIP3 (MO) CL:ANa40 (Cat. No. B7931). The antibody was developed with AlkPh APA Mouse Fab Ads Hu, Bov, Hor (Cat. No. A2179) and an NBT/BCIP substrate. Antibody dilution 4 μg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ANa40, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen 21.5 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 2-4 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells expressing human BNIP3. | |
| UniProt accession no. | Q12983  |
| Application: | Anti-BNIP3 antibody, Mouse monoclonal has been used in • western blotting • immunoblotting • immunocytochemistry • immunoprecipitation enzyme linked immunosorbent assay (ELISA) • immunofluorescence analysis |
| Application: | Rabbit monoclonal clone ANa40 anti-BNIP3 is used to tag BNIP3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of BNIP3 in the regulation of mitochondrial and non-mitochondrial mediated apoptosis. |
| Application: | The product is useful in ELISA, immunoblotting (21.5 kDa, apparent molecular mass in SDS-PAGE is approximately 30 and 60 kDa, for monomer and dimer, respectively), immunocytochemistry (4% formaldehyde), and immunoprecipitation. |
| Biochem/physiol Actions: | B-cell lymphoma 2 (BCL2) Interacting Protein 3 (BNIP3) functions without a typical Bcl-2 homology 3 (BH3) domain. It interacts withcell death abnormality gene 9 (CED-9). BNIP3 interaction with B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra-large (Bcl-XL) regulates apoptosis in mitochondria l and non-mitochondrial sites. Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction characterized by opening of the mitochondrial permeability transition (PT) pore, proton electrochemical gradient (Dym) suppression, and increased reactive oxygen species production. |
| Biochem/physiol Actions: | Rabbit monoclonal clone ANa40 anti-BNIP3 recognizes human BNIP3. The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-BNIP3 (mouse IgG2b isotype) is derived from the ANa40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a recombinant human BNIP3. B-cell lymphoma 2 (BCL2) Interacting Protein 3 (BNIP3) is a pro-apoptotic protein localized in mitochondria and belongs to Bcl-2 family. Its Bcl-2 homology 3 (BH3) domain is less conserved in mammalian cells and yeast. |
| Immunogen: | recombinant human BNIP3 (amino acids 1-163, BNIP3ΔTM2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |