Anti-Calcitonin Gene Related Peptide antibody produced in rabbit
SIGMA/C8198 - whole antiserum
Synonym: CGRP Antibody - Anti-Calcitonin Gene Related Peptide antibody produced in rabbit; Cgrp Antibody; Anti-CGRP
MDL Number: MFCD00162203
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: Translational control of nociception via 4E-binding protein 1. By: Khoutorsky, A., Bonin, R. P., et al. in Elife, 2015. PubMed ID: 26678009 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/12002), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry 4% paraformaldehyde perfusion-fixed, frozen sections of rat brain stained with 1:8,000 Anti-Calcitonin Gene Related Peptide antibody produced in Rabbit (Cat. No. C8198) followed by Anti-Rabbit IgG-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunofluorescence A transverse section of a Rat lumbar dorsal root ganglion stained with fluorescent markers to distinguish subpopulations of sensory neurons with distinct roles in the pain pathway. Large myelinated neurons and associated fibers are immunoreactive for neurofilament-200 (NF200, in green), while small neurons are divided into peptidergic (in red) and non-peptidercgic (in blue) nociceptors expressing calcitonin gene-related peptide (CGRP) or isolectin B4 (IB4), respectively. Small neurons can encode the intensity of a painful stimulus into firing frequencies, while large neurons are thought to principally mediate sensations of touch, pressure and proprioception. Submitted by Dr C. Tsantoulas, University of Cambridge
| antibody form | whole antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| UniProt accession no. | P06881  |
| Application: | Anti-Calcitonin Gene Related Peptide antibody produced in rabbit has been used in: • dot blot immunoassay • fluorescent labeling • confocal microscopy • immunohistofluorescence • epifluorescence imaging |
| Application: | Anti-Calcitonin Gene Related Peptide antibody produced in rabbit was used for immunohistochemistry of trigeminal ganglia cell cultures at a dilution of 1:200 and for immunocytochemistry of mouse lung slices at a dilution of 1:500. |
| Biochem/physiol Actions: | Anti-Calcitonin Gene Related Peptide reacts with CGRP (rat). Cross-reactivity is observed with CGRP (human) and β-CGRP (human). |
| Biochem/physiol Actions: | Calcitonin Gene Related Peptide is a G protein-coupled receptor that binds receptor-activity-modifyi |
| Biochem/physiol Actions: | CGRP is thought to act as a neurotransmitter or neuromodulator, particularly in the regulation of autonomic (e.g., cardiovascular, respiratory, gastrointestinal, taste, food intake and sleep) and limbic functions. CGRP is probably involved with substance P in mediating neurogenic inflammation and transmission of pain stimuli via the peripheral nervous system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calcitonin Gene Related Peptide (CGRP), a cyclic peptide, belongs to a small family of peptides encoded by the calcitonin/CGRP gene complex. The calcitonin/CGRP gene complex consists of at least two genes, encoding for two forms of CGRP: -CGRP (CGRP-I) and -CGRP (CGRP-II). CGRP is synthesized and co-localized with calcitonin in the parafollicular C-cells of the thyroid gland and medullary thyroid carcinoma. It is principally considered a neuropeptide widely distributed in the central and peripheral nervous systems. |
| Immunogen: | CGRP-KLH |
| Physical form: | Supplied as a liquid containing 0.1% sodium azide as a preservative |
| Preparation Note: | delipidized |
| Preparation Note: | For continuous use, store 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free"freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |