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Fetal Bovine Serum

SIGMA/F8192 - Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym: HI FBS; HI sera; HI serum; FBS; FCS; sera; serum

MDL Number: MFCD00132239
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-F8192-100ML 100 mL
$403.00
1/EA
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45-F8192-500ML 500 mL
$1560.00
1/EA
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biological source bovine fetus
composition Bovine IgG, ≤1 mg/mL
  Hemoglobin, ≤20 mg/dL
impurities ≤10 EU/mL endotoxin
origin Australia origin
product line FBS Classic
Quality Level 500 
shipped in dry ice
sterility sterile-filtered
storage temp. −20°C
technique(s) cell culture | hybridoma: suitable
  cell culture | mammalian: suitable
Analysis Note: • Endotoxin and hemoglobin tested
Analysis Note: • Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Application: FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.
General description: FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.
Preparation Note: Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352200

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