Fetal Bovine Serum
SIGMA/F8192 - Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma
Synonym: HI FBS; HI sera; HI serum; FBS; FCS; sera; serum
MDL Number: MFCD00132239
Product Type: Chemical
biological source | bovine fetus |
composition | Bovine IgG, ≤1 mg/mL |
Hemoglobin, ≤20 mg/dL | |
impurities | ≤10 EU/mL endotoxin |
origin | Australia origin |
product line | FBS Classic |
Quality Level | 500 |
shipped in | dry ice |
sterility | sterile-filtered |
storage temp. | −20°C |
technique(s) | cell culture | hybridoma: suitable |
cell culture | mammalian: suitable |
Analysis Note: | • Endotoxin and hemoglobin tested |
Analysis Note: | • Tested for the presence of bacteria, virus, and mycoplasma • Triple filtered with 0.1 micron membrane under aseptic conditions |
Application: | FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640. |
General description: | FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. |
Preparation Note: | Prepared by heating at 56°C for 30 minutes. This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |