Anti-GSDMD (126-138) antibody produced in rabbit
SIGMA/G7422 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Gasdermin D Antibody; Gasdermin D Antibody - Anti-GSDMD (126-138) antibody produced in rabbit; Gsdmd Antibody; Anti-DF5L; Anti-FKSG10; Anti-GSDMDC1; Anti-Gasdermin domain-containing protein 1
Product Type: Chemical
Immunoblotting Lysate of HEK-293T cells overexpressing human GSDMD was separated on SDS-PAGE, blotted with Anti-GSDMD (126-138) (Cat. No. G7422) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250
Immunofluorescence Anti-GSDMD (126-138): Cat. No. G7422: Immunofluorescence of HUVEC cells using GSDMD (126-138), Cat. No. G7422 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunoblotting Whole extract of human Jurkat cells was separated on SDS-PAGE and probed with Rabbit Anti-GSDMD (Cat. No. G7422). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + GSDMD immunizing peptide 3. Negative control: without first antibody
Chart Anti-GSDMD (126-138): Cat. No. G7422: Intensity analysis of Anti-GSDMD (126-138) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~55 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1:250-1:500 using human HEK-293T cells over-expressing human recombinant. GSDMDC1. |
| UniProt accession no. | P57764  |
| Application: | Anti-GSDMD (126-138) antibody produced in rabbit has been used in: • immunoblotting • CASP4/11 assay • cytokine enzyme?linked immunosorbent assay |
| Application: | Anti-GSDMD antibody produced in rabbit is suitable for western blot analysis at a working dilution of 1:500-1:2000. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | GSDMD (gasdermin D) have been suggested to function in the regulation of epithelial apoptosis. GSDMD is expressed in differentiating cells. It may act as a tumor suppressor. |
| Biochem/physiol Actions: | The absence of Gasdermin D (GSDMD), completely blocked LPS electroporation-triggered pyroptosis in studies performed on GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that caspase-4/11 specifically cleaves GSDMD after Asp275, results in gasdermin-N domain that bears intrinsic pyroptosis inducing activity. Furthermore, GSDMD-mediated pyroptosis participates in mature interleukin 1β (IL-1β) release without distressing its maturation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in upper gastrointestinal epithelium differentiating cells. |
| Immunogen: | synthetic peptide corresponding to amino acids 126-138 of human GSDMD |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |