Anti-Histone H3 antibody produced in rabbit
SIGMA/H0164 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti H3 Antibody; Histone H3 Antibody; Histone H3 Antibody - Anti-Histone H3 antibody produced in rabbit
MDL Number: MFCD01322157
Product Type: Chemical
Western Blotting JOURNAL CITATION: Depletion of cellular iron by curcumin leads to alteration in histone acetylation and degradation of Sml1p in Saccharomyces cerevisiae. By: Azad, G. K., Singh, V., et al. in PLoS One, 2013. PubMed ID: 23520547 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0059003), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2 μg/mL Anti-Histone H3 (N-terminal) antibody produced in Rabbit (Cat. No. H9289) (A). The antibody developed using Goat Anti-Rabbit IgG, FITC conjugate antibody, and 1:250 Anti-Histone H3 antibody produced in Rabbit (Cat. No. H0164) (B). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate antibody. Results Two Anti-Histone H3 antibodies, H9289 (A) and H0164 (B), target different regions of Histone H3 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Anti-Histone H3: Cat. No. H0164: Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded rat colon at 0.2 μg/mL.
Immunohistochemistry Localization of histone H3 in human breast carcinoma. Formalin-fixed paraffin-embedded tissue was stained with 20 μg/mL Anti-Histone H3 (Cat. No. H0164) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin®-HRP (×10 magnification).
Immunohistochemistry Anti-Histone H3: Cat. No. H0164: Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded rat pancreas at 0.2 μg/mL.
Immunohistochemistry Anti-Histone H3: Cat. No. H0164: Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded mouse testis at 0.2 μg/mL.
Immunohistochemistry Anti-Histone H3: Cat. No. H0164: Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded mouse brain at 0.2 μg/mL.
Immunofluorescence Nuclear localization of histone H3 in HeLa cells. Fixed cells were stained with 10 μg/mL Anti-Histone H3 (Cat. No. H0164), followed by Goat Anti-Rabbit IgG, FITC conjugate (×40 magnification). Actin staining with phalloidin rhodamine conjugate.
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Histone H3 (Cat. No. H0164) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. A431; 3. NIH3T3; 3. PC12; 4. A431. Antibody + immunizing histone H3 peptide
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 17 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | chicken, mouse, plant, Drosophila, rat, human, Xenopus |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells |
| western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line | |
| western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line | |
| UniProt accession no. | P68431  |
| Application: | Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone proteins are known to stabilize and regulate the nuclear DNA. Core histones, H2A, H2B, H3 and H4 aid the packaging of eukaryotic nuclear DNA into repeating nucleosome units while the linker histone, H1, binds to DNA between the nucleosomes. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation and methylation, ubiquitination and ADP ribosylation occur in histones, particularly on the N-terminal tail domains of histones, particularly of H3 and H4.These modifications dictate chromatin states and the gene expression. Phosphorylation of H3, mainly on Ser10 and Ser28, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Histone methylation, like acetylation, is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis ad heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9, and Lys27 are the preferred sites of methylation. Histone lysine residues can be mono-, di-, and tri-methylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure. |
| Immunogen: | synthetic peptide corresponding to amino acids 125-136 located at the C-terminus of human histone H3, conjugated to KLH. This sequence is identical in many species including rat, mouse, chicken, Xenopus, Drosophila, and plant histone H3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | Anti-Histone H3 specifically recognizes histone H3. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |