Heparinase I from Flavobacterium heparinum
SIGMA/H2519 - Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
Synonym: Heparin lyase I; Heparinase
CAS Number: 9025-39-2
MDL Number: MFCD00131317
Product Type: Chemical
Heparinase I, II, and III specificities: Heparan sulfate and heparin are complex heterogeneous mixtures of repeating disaccharide units consisting of a uronic acid (D-glucuronic or L-iduronic acid) and D-glucosamine or N-acetyl-D-glucosamine. Various degrees of sulfation occur (at O and/or N) on each monosacchiride unit, ranging from zero to tri-sulfation. In general, heparan is less sulfated than heparin.
| application(s) | diagnostic assay manufacturing |
| conjugate | conjugate (Glucosaminoglycan) |
| form | lyophilized solid |
| mol wt | 42.8 kDa |
| Quality Level | 200  |
| specific activity | ≥200 units/mg protein (enzyme + BSA) |
| storage temp. | −20°C |
| Application: | Heparinase I from Flavobacterium heparinum has been used: • in the digestion of plasma samples • to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia • in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells |
| Biochem/physiol Actions: | Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Unit Definition: | One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein (enzyme + BSA) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |