Heparinase I and III Blend from Flavobacterium heparinum
SIGMA/H3917 - lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA)
Synonym: Heparinase I and Heparinase III blend
Product Type: Chemical
Heparinase I, II, and III specificities: Heparan sulfate and heparin are complex heterogeneous mixtures of repeating disaccharide units consisting of a uronic acid (D-glucuronic or L-iduronic acid) and D-glucosamine or N-acetyl-D-glucosamine. Various degrees of sulfation occur (at O and/or N) on each monosacchiride unit, ranging from zero to tri-sulfation. In general, heparan is less sulfated than heparin.
| biological source | bacterial (Flavobacterium heparinum) |
| concentration | ≥200 unit/mg protein (enzyme + BSA) |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥200 units/mg protein |
| storage temp. | −20°C |
| Application: | Heparinase I and III Blend from Flavobacterium heparinum has been used in: • the digestion of heparan sulfate from ovine vitreous • human embryonic kidney cells • glycosaminoglycans from arterial tissues • P0 retinae digestion |
| Biochem/physiol Actions: | Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. |
| Biochem/physiol Actions: | Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides. |
| General description: | Heparinase is an inducible, non-extracellular heparin-degrading enzyme. Three types of heparinises are produced by Flavobacterium heparinum and contains specific sequences of heparin. |
| Other Notes: | Enzyme Commission Numbers: 4.2.2.7 Hep I and 4.2.2.8 Hep III |
| Packaging: | Sold on the basis of Heparinase I units |
| Unit Definition: | One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I. One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidney Heparan, Sulfate as substrate for heparinase III. One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |