Heparinase III from Flavobacterium heparinum
SIGMA/H8891 - Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)
Synonym: Heparin Lyase III; Heparitinase I; Heparitinase from Flavobacterium heparinum
CAS Number: 37290-86-1
MDL Number: MFCD00131319
Product Type: Chemical
Heparinase I, II, and III specificities: Heparan sulfate and heparin are complex heterogeneous mixtures of repeating disaccharide units consisting of a uronic acid (D-glucuronic or L-iduronic acid) and D-glucosamine or N-acetyl-D-glucosamine. Various degrees of sulfation occur (at O and/or N) on each monosacchiride unit, ranging from zero to tri-sulfation. In general, heparan is less sulfated than heparin.
| application(s) | diagnostic assay manufacturing |
| biological source | bacterial (Flavobacterium heparinum) |
| form | lyophilized powder |
| mol wt | 70.8 kDa |
| Quality Level | 200  |
| specific activity | ≥30 units/mg protein (enzyme + BSA) |
| storage temp. | −20°C |
| Application: | Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody. |
| Biochem/physiol Actions: | Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. |
| Biochem/physiol Actions: | Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins. |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Unit Definition: | One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥30 units/mg protein (enzyme + BSA) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.8 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |