Anti-EMD antibody produced in rabbit

SIGMA/HPA000609 - Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-Emerin antibody produced in rabbit

MDL Number: MFCD01633315
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-HPA000609-100UL	100 µL	$564.00 1/EAAdd To Favorites	Add To Favorites

Enhanced Validation-RNAi Knockdown Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe nos. 1 and 2, using Anti-EMD antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

antibody form	affinity isolated antibody
antibody product type	primary antibodies
biological source	rabbit
clone	polyclonal
conjugate	unconjugated
enhanced validation	RNAi knockdown Learn more about Antibody Enhanced Validation
form	buffered aqueous glycerol solution
immunogen sequence	ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY
product line	Prestige Antibodies^® Powered by Atlas Antibodies
Quality Level	100
shipped in	wet ice
species reactivity	rat, human, mouse
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunoblotting: 0.04-0.4 μg/mL
	immunofluorescence: 0.25-2 μg/mL
	immunohistochemistry: 1:500-1:1000
UniProt accession no.	P50402

Application:	Anti-EMD antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige .
Application:	Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper)
Biochem/physiol Actions:	EMD encodes a serine-rich nuclear membrane protein named emerin that belongs to the nuclear lamina-associated protein family. Emerin is involved in the formation of actin-based cortical network at the nuclear inner membrane. It regulates signalling pathways by interacting with β-catenin (transcription coactivator) and controlling its flux into the nucleus. It is necessary for HIV-1 infection in the chromatin engagement by viral cDNA before integration. It is involved in the linkage of centrosome to the outer nuclear membrane.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits:	Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments.
Immunogen:	Emerin recombinant protein epitope signature tag (PrEST)
Legal Information:	Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Linkage:	Corresponding Antigen APREST74058
Physical form:	Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203