Anti-LAMP1 antibody produced in rabbit
SIGMA/L1418 - affinity isolated antibody, buffered aqueous solution
Synonym: LAMP1 Antibody - Anti-LAMP1 antibody produced in rabbit; Lamp1 Antibody; Anti-CD107a; Anti-LAMPA; Anti-LGP120; Anti-
MDL Number: MFCD01864611
Product Type: Chemical
Immunofluorescence
Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 μg/mL Anti-LAMP1 (Cat. No. L1418) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 μg/mL Anti-LAMP1 (Cat. No. L1418) and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunofluorescence
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 μg/mL Anti-LAMP1 (Cat. No. L1418) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 μg/mL Anti-LAMP1 (Cat. No. L1418) and developed with Goat Anti-Rabbit IgG, FITC conjugate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~120 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HeLa, rat NRK, and mouse NIH3T3 cells |
| UniProt accession no. | P11279  |
| Application: | Anti-LAMP1-Cy3® antibody produced in rabbit has been used in western blotting and immunofluorescence. |
| Biochem/physiol Actions: | Lysosome-associated membrane protein 1 (LAMP1) with heavy glycosylation, may be important to protect the lysosomal membrane from proteolytic enzymes within lysosomes. |
| Biochem/physiol Actions: | The gene LAMP1 (lysosomal associated membrane protein 1) encodes a membrane glycoprotein that functions as an intracellular receptor. It is found to be expressed in the cytoplasm of several types of tumor cells and may be involved in tumor invasion. Lamp1 is crucial for perforin trafficking to the lytic granules and motility of these lytic granules. Its knockdown leads to inhibition of cytotoxicity of human natural killer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Lysosome-associated membrane protein 1 (LAMP1), also termed Igp120, is a heavily glycosylated lysosomal membrane protein of about 120 kDa. It consists of a ~40 kDa core polypeptide with O-linked and 18 asparagine-linked oligosaccharide side chains. LAMP1 protein contains a leader sequence, a large intralumenal region consisting of 2 homologous domains separated by a hinge region rich in proline and serine, a 24-amino acid transmembrane region, and a short cytoplasmic tail containing the lysosomal membrane targeting signal. LAMP1 is ubiquitously expressed and highly conserved. It localizes mainly to lysosomes although a small portion is detected on the cell surface. It was found that highly metastatic tumor cells express more LAMP molecules on the cell surface than poorly metastatic cells. |
| General description: | The gene LAMP1 (lysosomal-associated membrane protein 1) encodes a type I transmembrane protein that has a short cytoplasmic tail containing a lysosome-targeting signal of GYQTI(382)-COOH. The gene is mapped to human chromosome 13q34. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 405-416 of human LAMP1 with N-terminal added cysteine-glycine, conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Legal Information: | CAB-O-SIL is a registered trademark of Cabot Corp. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |