Anti-L1CAM antibody, Mouse monoclonal
SIGMA/L4543 - clone UJ127.11, purified from hybridoma cell culture
Synonym: L1CAM Antibody - Anti-L1CAM antibody, Mouse monoclonal; L1Cam Antibody; Anti-CAML1; Anti-L1 Cell Adhesion Molecule; Anti-MIC5; Anti-Neural Cell Adhesion Molecule L1
MDL Number: MFCD01864727
Product Type: Chemical
Western Blotting Whole extract of G361 human melanoma cells was separated on SDS-PAGE and probed with 0.25 μg/mL Anti-L1CAM antibody, Mouse monoclonal (Cat. No. L4543). The antibody was developed using Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 0.25 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | UJ127.11, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 220-240 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| microarray: suitable | |
| western blot: 0.25-0.5 μg/mL using cell extracts of G361 cells (human melanoma) | |
| UniProt accession no. | P32004  |
| Application: | Anti-L1CAM antibody has been used in immunohistochemistry. |
| Application: | Anti-L1CAM antibody, Mouse monoclonal has been used for: • immunohistochemistry • immunoprecipitation • immunoblotting • immunocytochemistry • flow cytometry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Biochem/physiol Actions: | L1 cell adhesion molecule (L1CAM) is critical for central nervous system (CNS) development in humans. This protein is responsible for many activities among them: neurite outgrowth, adhesion, fasciculation and myelination. These activities are mediated by the interaction of L1CAM with various members of the CAM protein family, extracellular matrix molecules and signaling receptors, through interactions that involve the extracellular domain of L1CAM. Mutations in the L1CAM gene may be linked to several brain disorders such as type 1 X-linked spastic paraplegia (SPG1), MASA syndrome and ACC (agenesis of corpus callosum). |
| Biochem/physiol Actions: | The gene L1CAM (L1 cell adhesion molecule) encodes a glycoprotein that functions in axon growth during development and axon bundling. It interacts with Schwann cells and axons, and mediates neuronal cell migration, and neuronal cell survival. Overexpression of this protein is associated with tumor progression. It has been found to mediate therapeutic resistance in ovarian and pancreatic cancer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-L1CAM antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma UJ127.11 produced by the fusion of mouse myeloma cells (P3- X63-Ag8-653 cells) and splenocytes from mice immunized with human fetal brain. L1 cell adhesion molecule (L1CAM) protein belongs to the family of cell adhesion molecules (CAM). |
| General description: | The gene L1CAM (L1 cell adhesion molecule) encodes a member of the immunoglobulin supergene family. The encoded protein contains a cytoplasmic intracellular domain, one single-pass transmembrane region and an extracellular domain containing six immunoglobulin- (Ig) and five fibronectin (Fn) III-like motifs. The gene is mapped to human chromosome Xq28. It contains 29 exons that encode a protein of 1257 amino acids containing a 19 amino acid signal peptide and a functional L1 protein of 1238 amino acids. It is mainly expressed in the nervous system. |
| Immunogen: | L1CAM from human fetal brain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |