Anti-BRD7 antibody, Rat monoclonal
SIGMA/SAB4200047 - clone BRM 2D3, purified from hybridoma cell culture
Synonym: Anti-BP75; Anti-Bromodomain containing 7; Anti-CELTIX1; Anti-NAG4; Monoclonal Anti-BRD7 antibody produced in rat
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1 μg/mL Rat Anti-BRD7 Clone: BRM 2D3 (Cat. No. SAB4200047). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. HeLa; 2. Jurkat; 3. NRK; 4. PC12; 5. Neuro-2A; 6. NS1; 7. MDBK; 8. MDCK; 9. COS7
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | BRM 2D3, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~80 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | rat, mouse, monkey, human, bovine, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.5-1.0 μg/mL using HeLa cells extract | |
| UniProt accession no. | Q9NPI1   |
| Application: | Monoclonal Anti-BRD7 antibody produced in rat has been used in several immunochemical techniques including • immunoblotting • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Bromodomain containing (BRD7) inhibits cell growth and cell cycle progression by transcriptional regulation of some cell cycle-related genes as well as some important molecules involved in ras/mitogen-activated protein kinase(MEK)/extracellular |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-BRD7 (rat IgG2a isotype) is derived from the hybridoma BRM 2D3 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a fusion protein expressing a fragment of human BRD7. Bromodomain containing (BRD7) is a novel bromodomain gene. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |