Anti-PSMA antibody, Mouse monoclonal
SIGMA/SAB4200257 - clone 107-1A4, purified from hybridoma cell culture
Synonym: PSMA Antibody - Monoclonal Anti-PSMA antibody produced in mouse; Psma Antibody; Monoclonal Anti-FOLATE HYDROLASE 1; Monoclonal Anti-FOLH1; Monoclonal Anti-GCP2; Monoclonal Anti-GLUTAMATE CARBOXYPEPTIDASE II; Monoclonal Anti-N-ACETYLATED ALPHA-LINKED ACIDIC DIPEPTIDASE 1; Monoclonal Anti-NAALAD; Monoclonal Anti-
Product Type: Chemical
Immunofluorescence LNCaP cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-PSMA Clone: 107-1A4 (Cat. No. SAB4200257). The antibody was developed using Goat Anti-Mouse IgG, ATTO 488 conjugate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 107-1A4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| immunocytochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: 5-10 μg/mL using methanol/acetone fixed LNCaP cells. |
| Application: | Monoclonal Anti-PSMA antibody produced in mouse has been used in: • enzyme linked immuno sorbent assay (ELISA) • immunocytochemistry • immunoprecipitation • flow cytometry |
| Biochem/physiol Actions: | Prostate-specific membrane antigen (PSMA), expressed in the brain, catalyzes the hydrolysis of N-acetyl-aspartyl glutamate to produce free glutamate. Elevated expression of the protein is associated with the development of prostate tumor. Mutations in PSMA leads to hyperhomocysteinemia. PSMA functions as a potential biomarker of prostate adenocarcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-PSMA (mouse IgG1 isotype) is derived from the hybridoma 107-1A4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Prostate-specific membrane antigen (PSMA) is an integral, non-shed, type 2 transmembrane glycoprotein belonging to the M28 peptidase family. |
| General description: | Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1 (FOLH1), is a 100kDa membrane glycoprotein, composed of 750 amino acids. PSMA gene with 19 exons and 18 introns is mapped to human chromosome 11p11–12. The encoded protein is detected in various type of tissues including kidney, prostate and brain. PSMA contains short cytoplasmic amino terminus, a single membrane-spanning domain and a large extracellular domain. |
| Immunogen: | prostate cancer LNCaP cells. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |