Anti-Kindlin-1 antibody, Mouse monoclonal
SIGMA/SAB4200465 - clone KN-4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-C20orf42; Monoclonal Anti-DTGCU2; Monoclonal Anti-FERMT1 URP1; Monoclonal Anti-KIND1; Monoclonal Anti-Kindlin-1 antibody produced in mouse; Monoclonal Anti-UNC112A; Monoclonal Anti-fermitin family member 1
Product Type: Chemical
Immunofluorescence SW-620 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 5 μg/mL Mouse Anti-Kindlin 1 Clone: KN-4 (Cat. No. SAB4200465). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting SW-48 cell extract was separated on SDS-PAGE and probed with Mouse Anti-Kindlin 1 Clone: KN-4 (Cat. No. SAB4200465). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 1 μg/mL antibody + 25 μ/mL Kindlin 1 immunizing peptide (human, 192-210) 3. Negative control
Immunoblotting Cell extracts were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-Kindlin 1 Clone: KN-4 (Cat. No. SAB4200465). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. CFB 2. BE 3. MDCK 4. BHK 5. LA4 6. MDBK 7. HEK-293T 8. COS-7 9. 4T1 10. HEPA-6 11. L929
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | KN-4, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa (isoform 4) |
| antigen ~60 kDa (isoform 2) | |
| antigen ~77 kDa (isoform 1) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using SW-620 cells |
| western blot: 1.0-2.0 μg/mL using SW-48 total cell extracts | |
| UniProt accession no. | Q9BQL6  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Kindlin-1 antibody has been used in immunohistochemistry, immunofluorescence staining and western blotting. |
| Biochem/physiol Actions: | Kindlin-1 protein, coded by FERMT1 gene modulates keratinocyte electrotaxis. It participates in the initiation of the integrin family of extracellular matrix receptors. FERMT1 maintains the lamellipodial protrusions at the time of electrotaxis. Mutations in kindlin-1 results in Kindler syndrome (KS). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Kindlin-1 is coded by FERMT1 (fermitin family member 1) gene. It is a focal adhesion protein, expressed in the skin and intestine. FERMT1 is located on human chromosome 20p.12.3. |
| Immunogen: | synthetic peptide corresponding to a sequence close to the N-terminus of human Kindlin-1, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |