Anti-IDH1 (R132H) antibody, Mouse monoclonal
SIGMA/SAB4200548 - clone HMab-1, purified from hybridoma cell culture
Synonym: Idh1 R132H Antibody; Idh1 R132H Antibody - Anti-IDH1 (R132H) antibody, Mouse monoclonal; Anti-IDCD; Anti-IDH; Anti-IDP; Anti-IDPC; Anti-Isocitrate dehydrogenase 1 (NADP+), soluble; Anti-
Product Type: Chemical
Immunoblotting Extract of HEK-293T cells overexpressing human IDH1(R132H) was separated on SDS-PAGE and probed with Mouse Anti-IDH1(R132H) Clone: HMab-1 (Cat. No. SAB4200548). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 16 μg/mL antibody 2. 8 μg/mL antibody 3. 4 μg/mL antibody 4. 2 μg/mL antibody 5. Negative control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | HMab-1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~43 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 4.0-8.0 μg/mL using extract of HEK-293T cells overexpressing IDH1R132H. |
| UniProt accession no. | O75874  |
| Application: | Anti-IDH1 (R132H) antibody has been used: • in immunoblotting • in immunohistochemistry • in western blotting • in cell cycle analysis and apoptosis assays • in chromatin immunoprecipitation (ChIP) assay • in in vitro migration assay |
| Biochem/physiol Actions: | Isocitrate dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate into α α -KG) using NADP as co-substrate. Mutations in IDH1 are specific to Arg132 (R132) and endow them with the function of generating 2-hydroxyglutarate (2HG) instead of α-KG. This product alters gene transcription through effects on DNA and histone methylation. Several IDH1 mutations exist, including R132H, R132C, R132S, R132G and R132L. Each may result in different tumor type with varied malignant progression. The most frequent known mutation (>90%) is the alteration of arginine to histidine (R132H). Hence, antibodies that recognize the IDH1R132H mutation can be useful for the diagnosis of mutation-bearing tumors like gliomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-IDH1 (R132H) antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma HMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to mutation R132H of human IDH1. A member of isocitrate dehydrogenase (IDH) family, IDH1, is the human cytoplasmic NADP-specific enzyme. Its subcellular localization was shown to be in both peroxisomes and the cytoplasm. |
| Immunogen: | peptide corresponding to mutation R132H of human IDH1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |