Anti-Bovine Serum Albumin antibody, Mouse monoclonal
SIGMA/SAB4200688 - clone BSA-33, purified from hybridoma cell culture
Synonym: Albumin bovine serum; BSA; Bovine albumin
Product Type: Chemical
Immunoblotting Fetal bovine serum was separated on SDS-PAGE and probed with Monoconal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. SAB4200688). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 1 μg/mL antibody 4. 2 μg/mL antibody 5. 1:1,000 B2901 antibody dilution 6. Negative Control: without first antibody
Immunoprecipitation Monoclonal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. SAB4200688) was used to immunoprecipitate Bovine Serum Albumin from 2 μL/test FCS (lanes 1-3) and from 2 μg/test BSA (lanes 5-6). Detection antibody: Rabbit Anti-BSA (Cat. No. SAB4200656), followed by Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/test antibody 2. 2 μg/test antibody 3. Negative control: 2 μg/test non-relevant antibody 4. Whole FCS 2 μL/lane 5. 1 μg/test antibody 6. 2 μg/test antibody 7. Whole BSA 2 μg/lane
ELISA Monoclonal Anti-Bovine Serum Albumin, Clone: BSA-33 (Cat. No. SAB4200688) was serially diluted for indirect ELISA using 5 μg/mL BSA for coating. The antibody was developed using Goat Anti-Mouse IgG (Fab specific)-Peroxidase (Cat. No. A9917) and a chromogenic substrate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BSA-33, monoclonal |
| concentration | ~1 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~70 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | sheep (highly reactive ), horse (less reactive ), dog (less reactive ), turkey (less reactive ), goat (highly reactive ) |
| storage temp. | −20°C |
| technique(s) | immunoblotting: 0.5-1 μg/mL using Fetal Bovine Serum. |
| immunoprecipitation (IP): 1-2 μg using Fetal Bovine Serum. | |
| indirect ELISA: 0.03-0.075 μg/mL using 5 μg/mL BSA for coating. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Serum albumin, the most abundant protein (30–50 mg/mL) in the plasma, is a multifunctional, non-glycosylated, negatively charged protein produced in the liver. It binds to a wide variety of lipophilic compounds such as metal ions, pharmaceuticals and metabolites. Albumin regulates osmotic pressure in the blood and has a role in the transport of fatty acids. |
| Immunogen: | Bovine Serum Albumin (BSA) |
| Other Notes: | In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Storage and Stability: | For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |