Anti-Emerin/EMD antibody produced in rabbit
SIGMA/SAB5701509
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using Emerin/EMD Rabbit mAb (SAB5701509) at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using Emerin/EMD Rabbit mAb (SAB5701509) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using Emerin/EMD Rabbit mAb (SAB5701509) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Emerin/EMD Rabbit mAb (SAB5701509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | monoclonal |
| concentration | 0.6 mg/mL |
| form | liquid |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P50402  |
| General description: | Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A synthesized peptide derived from human Emerin/EMD |
| Physical form: | Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |
| Storage Temp. | −20°C |