ZORBAX Poroshell 300Å HPLC Columns
By Agilent Technologies
Features:
- High-resolution separations of biomolecules with unique particle design
- High efficiency and recovery with proteins (up to 1,000 kDa) and monoclonal antibodies
- Achieve long lifetime at low pH with Poroshell 300SB; at high pH with 300Extend-C18
- Optimize recovery and selectivity with four different bonded phases 300SB-C18, 300SB-C8, 300SB-C3 and 300Extend-C18
ZORBAX Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow-diffusing analytes, but Poroshell columns use a unique particle made with a thin layer of porous silica on a solid core of silica. This reduces the diffusion distance for proteins, making for practical rapid HPLC separations of peptides and proteins up to 500 1,000 kDA. Poroshell columns bonded with StableBond bonded phases provide excellent stabiltiy and selectivity choices with TFA and formic acid mobile phases. The Poroshell 300Extend-C18 column can be used from pH 2 to pH 10 for unique separations. These columns can be used for analytical protein separations as well as LC/MS separations.