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Diethyl pyrocarbonate

ALDRICH/159220 - 96% (NT)

Synonym: DEP; DEPC; Diethyl dicarbonate; Diethyl oxydiformate; Ethoxyformic acid anhydride

CAS Number: 1609-47-8
Empirical Formula (Hill Notation): C6H10O5
Molecular Weight: 162.14
EC Number: 216-542-8
MDL Number: MFCD00009106
Linear Formula: O(COOC2H5)2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-159220-5G 5 g
 $55.60
1/EA		 Add To Favorites
45-159220-25G 25 g
 $136.00
1/EA		 Add To Favorites
45-159220-100G 100 g
 $303.00
1/EA		 Add To Favorites

 

assay 96% (NT)
bp 93-94 °C/18 mmHg (lit.)
density 1.101 g/mL at 25 °C (lit.)
form liquid
functional group carbonate
InChI 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key FFYPMLJYZAEMQB-UHFFFAOYSA-N
Quality Level 200 
refractive index n20/D 1.398 (lit.)
shipped in wet ice
SMILES string CCOC(=O)OC(=O)OCC
storage temp. 2-8°C
Application: Diethyl pyrocarbonate was used as:
• nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
• an antimicrobial additive
• ribonuclease inhibitor
• histidine residue modifier
• reagent for conversion of imines to carbamates
Application: Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
Biochem/physiol Actions: Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
General description: Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.
Packaging: 25, 100 g in poly bottle
Packaging: 5 g in glass bottle
Symbol GHS07  GHS07
Signal word Warning
Hazard statements H302
Precautionary statements P301 + P312 + P330
Hazard Codes Xn
Risk Statements 22-36/37/38
Safety Statements 26-36
WGK Germany WGK 3
Flash Point(F) 156.2 °F - closed cup
Flash Point(C) 69 °C - closed cup
Purity 96% (NT)
bp 93-94 °C/18 mmHg (lit.)
Density 1.101 g/mL at 25 °C (lit.)
Refractive Index n20/D 1.398 (lit.)
Storage Temp. 2-8°C
UNSPSC 12352100

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