Diethyl pyrocarbonate
ALDRICH/159220 - 96% (NT)
Synonym: DEP; DEPC; Diethyl dicarbonate; Diethyl oxydiformate; Ethoxyformic acid anhydride
CAS Number: 1609-47-8
Empirical Formula (Hill Notation): C6H10O5
Molecular Weight: 162.14
EC Number: 216-542-8
MDL Number: MFCD00009106
Linear Formula: O(COOC2H5)2
Product Type: Chemical
| assay | 96% (NT) |
| bp | 93-94 °C/18 mmHg (lit.) |
| density | 1.101 g/mL at 25 °C (lit.) |
| form | liquid |
| InChI | 1S/C6H10O5/c1-3-9-5(7)11- |
| InChI key | FFYPMLJYZAEMQB-UHFFFAOYSA |
| Quality Level | 200  |
| refractive index | n |
| shipped in | wet ice |
| SMILES string | CCOC(=O)OC(=O)OCC |
| storage temp. | 2-8°C |
| Application: | Diethyl pyrocarbonate was used as: • nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues • an antimicrobial additive • ribonuclease inhibitor • histidine residue modifier • reagent for conversion of imines to carbamates |
| Application: | Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. |
| Biochem/physiol Actions: | Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. |
| General description: | Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied. |
| Packaging: | 25, 100 g in poly bottle |
| Packaging: | 5 g in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P301 + P312 + P330 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 156.2 °F - closed cup |
| Flash Point(C) | 69 °C - closed cup |
| Purity | 96% (NT) |
| bp | 93-94 °C/18 mmHg (lit.) |
| Density | 1.101 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352100 |