Resazurin sodium salt
ALDRICH/199303 - certified by the Biological Stain Commission
Synonym: 7-
CAS Number: 62758-13-8
Empirical Formula (Hill Notation): C12H6NNaO4
Molecular Weight: 251.17
EC Number: 263-718-5
MDL Number: MFCD00005036
Linear Formula: C12H6NNaO4
Product Type: Chemical
λmax | 380 nm (2nd) |
598 nm | |
application(s) | diagnostic assay manufacturing hematology histology |
composition | Dye content, 75% |
form | powder |
grade | certified by the Biological Stain Commission |
InChI | 1S/C12H7NO4.Na/c14-7-1-3- |
InChI key | IVGPGQSSDLDOLH-UHFFFAOYSA |
Quality Level | 200 |
SMILES string | [Na+].[O-]c1ccc2c(OC3=CC( |
solubility | 1 M NH4OH: 1 mg/mL |
storage temp. | room temp |
Application: | For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). |
Application: | Resazurin is mostly used as a biological redox indicator. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red). Its applications include: • the measurement of metabolic activity and the proliferation of living cells • the detection of bacterial reduction in milk • in viability assays as a component of culture media for the detection of viable bacteria • to determine the number of motile sperm in sheep semen • to assay lactate dehydrogenase • as an analytical reagent to quantify L-glutamate |
General description: | Resazurin is a small N-oxide derivative of dihydroxyphenoxazone. It is a non-ionic dye containing a small and planar chromophore. It is a redox indicator and changes from bluish violet through pink to colorless upon reduction. It is also used as a pH indicator for Ph 3.5 to 6.5 wherein it changed from orange to bluish violet. It is commercially available as resazurin sodium salt, which is also known as 3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt. It forms the red, fluorescent hydroxyphenoxazone resorufin on reduction and colorless hydroresorufin on further reduction. |
Packaging: | 1, 5, 25 g in glass bottle |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H315 - H319 - H335 |
Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | room temp |
UNSPSC | 12171500 |