Azure II eosinate
ALDRICH/200220 - powder
Synonym: Azure II-eosin; Methylene blue; eosinate of Azure II
CAS Number: 53092-85-6
MDL Number: MFCD00084753
Product Type: Chemical
ATR-IR Spectra for Azure II eosinate.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 200220-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 200220-25G
| λmax | 524 nm (2nd) |
| 647 nm | |
| Amax | ≥0.50 at 645-655 nm in methanol at 0.005 g/L |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark green |
| form | powder |
| InChI | 1S/C20H8Br4O5.C16H18N3S.C |
| InChI key | CSDDIABEPMTRIP-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | [Na+].[Na+].[Cl-].[Cl-].C |
| solubility | soluble, hazy to turbid |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Azure II Eosinate is used for human-medical cell staining and serves the purpose of the investigation of sample material of human origin (by fixing, embedding where necessary, staining, counterstaining, mounting) in hematological and clinico-cytological specimen materials, for example smears of whole blood and bone marrow. |
| Biochem/physiol Actions: | The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azure B with DNA. Both dyes assemble to an Eosin Y- Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. |
| Features and Benefits: | Chief ingredient of Giemsa Stain. |
| General description: | Azure II Eosinate is the eosinate of the dye Azure II which is a mixture of the dyes methylene blue and azure B in equal proportions. This compound is a key ingredient in Giemsa stain, which is widely utilized for staining blood elements and facilitating the visualization of cellular components under a microscope. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |  GHS05 |
| Signal word | Danger |
| Hazard statements | H318 |
| Precautionary statements | P280 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 41 |
| Safety Statements | 22-26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |