4′,5′-Dibromofluorescein
ALDRICH/216720 - Dye content 95 %
Synonym: Solvent Red 72
CAS Number: 596-03-2
Empirical Formula (Hill Notation): C20H10Br2O5
Molecular Weight: 490.10
EC Number: 209-876-0
MDL Number: MFCD00005042
Linear Formula: C20H10Br2O5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 216720-5G
| ε (extinction coefficient) | ≥5000 at 445-451 nm |
| ≥50000 at 227-233 nm | |
| λmax | 450 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | >95% (TLC) |
| composition | Dye content, 95% |
| form | powder |
| InChI | 1S/C20H10Br2O5/c21-15-13( |
| InChI key | ZDTNHRWWURISAA-UHFFFAOYSA |
| mp | 270-273 °C (lit.) |
| Quality Level | 100  |
| SMILES string | Oc1ccc2c(Oc3c(Br)c(O)ccc3 |
| storage temp. | room temp |
| Application: | 4′,5′-Dibromofluorescein has been used to stain proteins in 1-D and 2-D sodium dodecyl sulfatepolyacrylamide gel electrophoresis SDS-PAGE. This method is rapid, economical, and highly sensitive in comparison with other proteomic research staining methods. |
| General description: | 4′,5′-Dibromofluorescein (DBF) is a fluorone dye. Fluorone dyes are hydroxyl-xanthene dyes (homologues of fluorescein) and have been the most widely used class of organic fluorophores for labeling and sensing biomolecules. DBF is routinely used as a ligand for studying the properties of protein using spectral analysis. |
| Packaging: | 5 g in glass bottle |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H301 |
| Precautionary statements | P301 + P310 |
| Hazard Codes | T |
| Risk Statements | 25 |
| Safety Statements | 45 |
| RIDADR | UN 2811 6.1 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (TLC) |
| mp | 270-273 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 45370:1 |
| UNSPSC | 12171500 |