Sulforhodamine B
ALDRICH/230162 - Dye content 75 %
Synonym: Acid Red 52; Sulforhodamine B monosodium salt
CAS Number: 3520-42-1
Empirical Formula (Hill Notation): C27H29N2NaO7S2
Molecular Weight: 580.65
EC Number: 222-529-8
MDL Number: MFCD00010180
Linear Formula: C27H29N2NaO7S2
Product Type: Chemical
ε (extinction coefficient) | ≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L |
λmax | 554 nm |
application(s) | diagnostic assay manufacturing hematology histology |
assay | ≥73% (HPLC) |
color | brown to very dark brown |
green to very dark green | |
composition | Dye content, 75% |
form | powder |
InChI | 1S/C27H30N2O7S2.Na/c1-5-2 |
InChI key | SXQCTESRRZBPHJ-UHFFFAOYSA |
Quality Level | 100 |
SMILES string | [Na+].CCN(CC)c1ccc2c(OC3= |
solubility | methanol: 1 mg/mL |
storage temp. | room temp |
Application: | Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay. |
General description: | Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination. |
Packaging: | 1, 5 g in glass bottle |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 2 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥73% (HPLC) |
Storage Temp. | room temp |
Colour Index Number | 45100 |
UNSPSC | 12171500 |