Trypan Blue
ALDRICH/302643 - ≥80% (HPLC), Dye content 60 %
Synonym: Direct blue 14
CAS Number: 72-57-1
Empirical Formula (Hill Notation): C34H24N6Na4O14S4
Molecular Weight: 960.81
EC Number: 200-786-7
MDL Number: MFCD00003969
Linear Formula: C34H24N6O14S4Na4
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 302643-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 302643-25G
| ε (extinction coefficient) | ≥60000 at 603-607 nm in methanol at 0.01 g/L |
| 60000 at 603-607 nm in methanol | |
| λmax | 607 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥80% (HPLC) |
| color | dark green to brown, or Brown-Black |
| composition | Dye content, 60% |
| form | powder |
| InChI | 1S/C34H28N6O14S4.4Na/c1-1 |
| InChI key | GLNADSQYFUSGOU-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| SMILES string | [Na+].[Na+].[Na+].[Na+].C |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| Application: | Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells. |
| Biochem/physiol Actions: | Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells. This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm. |
| Biochem/physiol Actions: | Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay: • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution. • to stain plant tissue in order to visualize cell death. |
| General description: | Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. |
| Packaging: | 25, 100 g in glass bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H350 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 45 |
| Safety Statements | 53-45 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| Purity | ≥80% (HPLC) |
| mp | >300 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 23850; 23850 |
| UNSPSC | 12171500 |