Diethyl pyrocarbonate
ALDRICH/40718 - 96% (NT)
Synonym: DEP; DEPC; Diethyl dicarbonate; Diethyl oxydiformate; Ethoxyformic acid anhydride
CAS Number: 1609-47-8
Empirical Formula (Hill Notation): C6H10O5
Molecular Weight: 162.14
EC Number: 216-542-8
MDL Number: MFCD00009106
Linear Formula: O(COOC2H5)2
Product Type: Chemical
| assay | 96% (NT) |
| bp | 93-94 °C/18 mmHg (lit.) |
| color | APHA: ≤20 |
| density | 1.101 g/mL at 25 °C (lit.) |
| 1.121 g/mL at 20 °C | |
| form | liquid |
| InChI | 1S/C6H10O5/c1-3-9-5(7)11- |
| InChI key | FFYPMLJYZAEMQB-UHFFFAOYSA |
| refractive index | n |
| n |
|
| shipped in | wet ice |
| SMILES string | CCOC(=O)OC(=O)OCC |
| solubility | 95% ethanol: 4.5 g/10 mL, clear, colorless |
| storage temp. | 2-8°C |
| Application: | Diethyl pyrocarbonate may be used in the following studies: • For the modification of histidyl residues in proteins. • As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues. • For the modification of linear and supercoiled plasmid DNAs. • As a chemical probe to investigate the secondary structure in negatively supercoiled DNA. • For cabethoxylation of histidine residues of actin. |
| Application: | Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. |
| Biochem/physiol Actions: | Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation. |
| General description: | Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues. |
| Packaging: | 25, 100 mL in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P301 + P312 + P330 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NA 1993 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 156.2 °F - closed cup |
| Flash Point(C) | 69 °C - closed cup |
| Purity | 96% (NT) |
| bp | 93-94 °C/18 mmHg (lit.) |
| Density | 1.121 g/mL at 20 °C; 1.101 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352100 |