ISOGRO®-13C,15N,D Powder -Growth Medium
ALDRICH/608297 - 98 atom % 15N, 97-99 atom % D, 99 atom % 13C
MDL Number: MFCD03099824
Product Type: Chemical
Cardiac troponin cTnC(1-89) in pLysS. Cells grown at 37 °C in shaker flasks. Graph shows significant decrease in induction time when ISOGRO® used as supplement to minimal media.
| form | solid |
| isotopic purity | 97-99 atom % D |
| 98 atom % 15N | |
| 99 atom % 13C | |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | bio NMR: suitable |
| protein expression: suitable |
| Application: | ISOGRO®-13C,15N,D Powder -Growth Medium has been used for the generation of isotopically labeled recombinant tau to identify multiple phosphorylations in tau by NMR (nuclear magnetic resonance) spectroscopy. |
| General description: | ISOGRO® media is required for overcoming the growth limitations of minimal media. ISOGRO products are lysates of algae grown with stable isotopes (13C, 15N, and/or D). ISOGRO®-13C,15N,D powder -growth medium gives uniform labeling for protein expression NMR (nuclear magnetic resonance) studies. A typical algal lysate (ISOGRO medium) contains: 30% salts, 3% water, 2% glucose and 65% amino acids/peptides. |
| Legal Information: | ISOGRO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 1 g in glass bottle |
| Packaging: | This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service . |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |