PTAD-Alkyne
ALDRICH/794236 - 97%
Synonym: 4-
CAS Number: 1313211-51-6
Empirical Formula (Hill Notation): C11H9N3O3
Molecular Weight: 231.21
MDL Number: MFCD24386394
Linear Formula: C11H9N3O3
Product Type: Chemical
| assay | 97% |
| form | solid |
| InChI | 1S/C11H9N3O3/c1-2-7-17-9- |
| InChI key | IHJYYZNVZZKSCU-UHFFFAOYSA |
| mp | 176-181 °C |
| Quality Level | 100  |
| SMILES string | O=C1NNC(=O)N1c2ccc(OCC#C) |
| storage temp. | 2-8°C |
| Application: | Note: PTAD-Alkyne must be first activated by stirring in a 1:0.98 molar ratio with 1,3-dibromo-5,5-dimethylh General Procedure for Protein Modification with PTAD. Part 1: PTAD activation Mix together 1:0.98 molar equivalents of unactivated PTAD to 1,3-dibromo-5,5-dimethylh Color change is observed from colorless/pale yellow to deep red (approximately 5 min of mixing). After the solution turns red, store the now activated reagent on ice and use for protein modification within 30 min. Part 2: Protein modification Add protein solution in mixed phosphate/Tris buffer or Tris buffer (pH should be 6 - 9) to the eppendorf tube (or other vial) containing the activated PTAD reagent prepared above and mix gently at room temperature for up to 30 min. Preferably use 10-fold molar excess of reagent relative to protein. Use protein at a minimum concentration of 1 mg/ml (higher concentrations are preferred for enhanced labeling). Remove excess unreacted PTAD by gel filtration. |
| General description: | PTAD-Alkyne is a selective bioconjugation reagent for the modification of tyrosine residues with a terminal alkyne. Subsequent modification can then be afforded via the copper catalyzed azide/alkyne click chemistry reaction with an azide containing compound or biomolecule. This reagent has been shown to selectively introduce poly(ethylene glycol) or PEG chains onto proteins with surface exposed tyrosine residues. PTAD-Alkyne has also been used in the formation of antibody-drug conjugates. This reagent is compatible with different buffer systems such as PBS, Tris and mixed PBS/Tris buffer (preferred). The linkage with tyrosine has been shown to be stable to pH and temperature extremes as well as blood plasma. |
| Packaging: | 500 mg in amber glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 97% |
| mp | 176-181 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352106 |