APN-Maleimide
ALDRICH/806536
Synonym: 3-
Empirical Formula (Hill Notation): C13H6N2O2
Molecular Weight: 222.20
Linear Formula: C13H6N2O2
Product Type: Chemical
| form | powder |
| InChI | 1S/C13H6N2O2/c14-9-1-2-10 |
| InChI key | CHKKXKRQICWZFF-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | N#CC#CC1=CC=C(N2C(C=CC2=O |
| storage temp. | 2-8°C |
| Application: | APN-Maleimide is a bifunctional crosslinker for thiol-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. Due to kinetic resolution the first thiol reacts exclusively with the maleimide residue producing the protein-APN conjugate. This conjugate can be readily coupled with thiol-containing molecules. |
| Packaging: | 50 mg in glass bottle |
| Preparation Note: | Standard protein labeling procedure (cysteine labeling). • Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration. • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue). • Incubate at room temperature for 10 minutes. • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration. • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours. *Note: avoid thiol-containing buffers. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H315 - H319 - H335 |
| Precautionary statements | P301 + P312 + P330 - P302 + P352 - P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352111 |